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Pregled bibliografske jedinice broj: 70350

Biotinylated indoles as probes for indole-binding proteins


Dolušić, Eduard; Kowalczyk, Mariusz; Magnus, Volker; Sandberg, Göran; Normanly, Jennifer
Biotinylated indoles as probes for indole-binding proteins // Bioconjugate chemistry, 12 (2001), 2; 152-162 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 70350 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Biotinylated indoles as probes for indole-binding proteins

Autori
Dolušić, Eduard ; Kowalczyk, Mariusz ; Magnus, Volker ; Sandberg, Göran ; Normanly, Jennifer

Izvornik
Bioconjugate chemistry (1043-1802) 12 (2001), 2; 152-162

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bifunctional probe; indole; biotin; indole-binding protein; tryptophanase; lysozyme; serum albumin; avidin-enzyme conjugate

Sažetak
Biotinylated indoles were prepared for application as bifunctional probes for the detection of indole-binding proteins which participate in the life processes of humans, animals, plants, and bacteria. The indole nucleus was functionalized, at ring positions 3, 5, or 6, by attachment of a 2-aminoethyl group, which was then coupled to the carboxyl moiety of biotin, via a spacer composed of 3 or 4 concatenated beta-alanine residues. The constructs thus obtained were able to inhibit tryptophanase activity, similarly to indole in a concentration-dependent manner. They also bound strongly to lysozyme and weakly to bovine and human serum albumins, in accordance with the known affinities of these proteins for indole and 3-(2-aminoethyl)indole (tryptamine). The biotin end of the protein-bound bifunctional probes could then be detected by coupling to (strept)avidin conjugated to alkaline phosphatase or horseradish peroxidase, followed by incubation with substrates which are converted by these enzymes to intensely colored or chemiluminescent products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
00981010

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Eduard Dolušić (autor)

Avatar Url Volker Magnus (autor)


Citiraj ovu publikaciju

Dolušić, Eduard; Kowalczyk, Mariusz; Magnus, Volker; Sandberg, Göran; Normanly, Jennifer
Biotinylated indoles as probes for indole-binding proteins // Bioconjugate chemistry, 12 (2001), 2; 152-162 (međunarodna recenzija, članak, znanstveni)
Dolušić, E., Kowalczyk, M., Magnus, V., Sandberg, G. & Normanly, J. (2001) Biotinylated indoles as probes for indole-binding proteins. Bioconjugate chemistry, 12 (2), 152-162.
@article{article, year = {2001}, pages = {152-162}, keywords = {bifunctional probe, indole, biotin, indole-binding protein, tryptophanase, lysozyme, serum albumin, avidin-enzyme conjugate}, journal = {Bioconjugate chemistry}, volume = {12}, number = {2}, issn = {1043-1802}, title = {Biotinylated indoles as probes for indole-binding proteins}, keyword = {bifunctional probe, indole, biotin, indole-binding protein, tryptophanase, lysozyme, serum albumin, avidin-enzyme conjugate} }
@article{article, year = {2001}, pages = {152-162}, keywords = {bifunctional probe, indole, biotin, indole-binding protein, tryptophanase, lysozyme, serum albumin, avidin-enzyme conjugate}, journal = {Bioconjugate chemistry}, volume = {12}, number = {2}, issn = {1043-1802}, title = {Biotinylated indoles as probes for indole-binding proteins}, keyword = {bifunctional probe, indole, biotin, indole-binding protein, tryptophanase, lysozyme, serum albumin, avidin-enzyme conjugate} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Chemical Abstracts





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