engleski

Antimicrobial efficacy of Er, Cr:YSGG laser- activated irrigation compared to passive ultrasonic irrigation and RinsEndo against intracanal Enterococcus faecalis population

The aim of this study was to evaluate the antibacterial efficacy of active irrigation techniques: Er, Cr:YSGG laser-activated irrigation (LAI), passive ultrasonic irrigation (PUI), RinsEndo®, and conventional syringe irrigation against intracanal Enterococcus faecalis (E. faecalis). Root canals of 100 human extracted teeth were instrumented, sterilized in plasma, contaminated with E. faecalis and incubated for ten days. The samples were randomly distributed into five experimental groups (n=20 each): I: LAI by Er, Cr:YSGG (1, 25W, 20Hz) for 4x5 s ; II. Passive ultrasonic irrigation (PUI) for 60 s ; III. RinsEndo® system for 60 s, IV. 30-gauge syringe irrigation for 60 s, and one positive control group (n=10). In all experimental groups, 2.5% sodium hypochlorite (NaOCl) was used as an irrigant while the positive controls were rinsed with saline. The root canals were sampled by flushing with saline solution before and after the treatments, serially diluted and cultured. The presence or the absence of E. faecalis in the root canals was checked by polymerase chain reaction. There were no difference between three active irrigation techniques (P > 0.05), which were more effective than the conventional syringe irrigation (P<0.001). The results showed that the LAI, the PUI and the RinsEndo® were equally effective against two weeks old E. faecalis population. The LAI generated more negative bacterial samples which warrants further investigations.

disinfection; Enterococcus faecalis; laser; RinsEndo; root canal; ultrasonic

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