engleski

Genetic diversity of Dalmatian sage (Salvia officinalis L.) as assessed by RAPD markers

Dalmatian or common sage (Salvia officinalis L.) is an outcrossing plant species native to East Adriatic coast. Random Amplified Polymorphic DNA markers (RAPD) were used to analyze genetic diversity and structure of ten natural populations from the East-Adriatic coastal region. Th e highest genetic diversity was found in populations from the central and south Dalmatia, while the highest frequency down-weighted marker values were found in the northernmost populations and the southern most inland population. Although analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, highly significant FST values suggested the existence of genetic differentiation among populations. By assuming Hardy-Weinberg equilibrium within populations, the calculated FST value among population was moderate. Bayesian model-based clustering method revealed that at K = 2 all individuals belonging to two northern populations were assigned to a separate cluster from the individuals belonging to the rest of the population. At K = 3, the newly formed cluster grouped the majority of individuals belonging to populations from central Dalmatia. Th e high correlation between matrices of genetic and geographical distances showed that isolation by distance may play a considerable role in overall structuring of the genetic diversity.

Salvia ; population genetics ; RAPDs ; genetic structure ; isolation by distance

Croatian Science Foundation Project No 09.01/246: Epigenetic vs. genetic diversity in natural plant populations: A case study of Croatian endemic Salvia species