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Arachidonic and docosahexaenoic acid in the blood of a mother and umbilical vein in diabetic pregnant women


Berberović, Edina; Ivanišević, Marina; Horvatiček, Marina; Delaš, Ivančica; Djelmiš, Josip
Arachidonic and docosahexaenoic acid in the blood of a mother and umbilical vein in diabetic pregnant women // XXIV European Congress of Perinatal Medicine
Firenca, Italija, 2014. (poster, međunarodna recenzija, sažetak, ostalo)


Naslov
Arachidonic and docosahexaenoic acid in the blood of a mother and umbilical vein in diabetic pregnant women

Autori
Berberović, Edina ; Ivanišević, Marina ; Horvatiček, Marina ; Delaš, Ivančica ; Djelmiš, Josip

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
XXIV European Congress of Perinatal Medicine / - , 2014

Skup
XXIV European Congress of Perinatal Medicine

Mjesto i datum
Firenca, Italija, 04.-07.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Arachidonic acid; docosahexaenoic acid; type 1 diabetes; pregnancy

Sažetak
Introduction. The essential long-chain polyunsaturated fatty acid, arachidonic and docosahexaenoic acid are important for fetal growth and development. Fetal concentration of AA and DHA depends on the mother’s concentration, i.e. on their ingestion by food. Previous studies showed that type 1 diabetes is associated with low AA and DHA levels of pregnant women. The aims of the study were: 1. To determine total FAs concentration in the maternal vein serum and in the umbilical vein serum in pregnant women suffering from T1DM and Control group ; 2. To determine and analyze AA and DHA concentrations and percentages (%) in relation to the total FAs concentration in the mother’s vein serum and the umbilical vein serum in pregnant women suffering from T1DM compared to the Control group. Methods. The study included 63 pregnant women, 32 suffering from T1DM and 31 from Control group. Samples of a mother’s vein blood and the umbilical vein blood were taken immediately after the birth. The total lipid extraction was carried out by the mixture of chloroform: methanol solvent: a method of increasing polarity according to Folch. Heptadecanoic acid was used as internal standard in order to determine the exact proportion of total lipids. Fatty acids from lipid extracts were converted to methyl esters by transesterification with methanolic HCl (ref: ISO standard) and their composition analyzed by GC-MS on Varian 3400 (Varian, USA) equipped with Saturn II ion trap mass spectrometer operating in the electron impact (EI) mode. Results. There was statistically significant difference in the total FAs concentration in the maternal vein serum and the umbilical vein serum between the two groups (p<0.001). There was a statistically significant higher concentration of total FAs in the maternal and umbilical vein serum of the diabetic group. Statistically significant difference of AA and DHA concentrations in the maternal vein serum were found between two groups (AA: p<0.001 ; DHA: p<0.001). Significantly higher concentrations of AA and DHA were found in the umbilical vein serum in T1DM (AA: p<0.01 ; DHA: p<0.05). Higher AA and DHA concentrations were found in the maternal vein serum compared to an umbilical vein serum of the diabetic group. Conclusion. Our study found no difference in AA and DHA percentages in the maternal or in the umbilical vein serum of diabetic pregnant women. Despite of T1DM, a good metabolic control leads to insignificant changes in the AA and DHA levels in diabetic pregnancy. Further studies are needed to understand the mechanism of diabetic pregnancy and its influence on biochemical abnormality and its implications for fetal development.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti