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Pregled bibliografske jedinice broj: 700851

Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats


Kalasz, Judit; Pasztor, Toth; Bodi, Beata; Fagyas, Miklos, Toth, Attila; Pal, Bgattoa H.; Vari Sandor G.; Balog, Marta; Blažetić, Senka; Heffer, Marija; Papp, Zoltan; Borbely, Attila
Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats // Croatian medical journal, 55 (2014), 3; 239-249 doi:10.3325/cmj.2014.55.239 (međunarodna recenzija, članak, znanstveni)


Naslov
Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats

Autori
Kalasz, Judit ; Pasztor, Toth ; Bodi, Beata ; Fagyas, Miklos, Toth, Attila ; Pal, Bgattoa H. ; Vari Sandor G. ; Balog, Marta ; Blažetić, Senka ; Heffer, Marija ; Papp, Zoltan ; Borbely, Attila

Izvornik
Croatian medical journal (0353-9504) 55 (2014), 3; 239-249

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Acute stress; ovariectomy; progesteron; cardiomyocytes; left ventriculi; rat

Sažetak
To assess how ovarian-derived sex hormones (in par ticular progesterone) modify the effects of ingle acute stress on the mechanical and biochemical properties of left ventricular cardiomyocytes in the rat. Non-ovariectomized (control, n=8) and ovariectomized (OVX, n=8) female rats were kept under normal conditions or were exposed to stress (control-S, n=8 and OVX-S, n=8). Serum progesterone levels were measured using a chemiluminescent immunoassay. Left ventricular myocardial samples were used for isometric force measurements and protein analysis. Ca2+ dependent active force (F active), Ca2+ independent passive force (F passive), and Ca 2+ sensitivity of force production were determined in single, mechanically isolated, permeabilized cardiomyocytes. Stress and ovariectomy induced alterations in myofilament proteins (myosin- binding protein C [MyBP-C], troponin I [TnI], and titin) were analyzed by sodium dodecyl sulfate gel electrophoresis using protein and phosphoprotein stainings. Serum progesterone levels were significantly in creased in stressed rats (control-S, 35.6±4.8 ng/mL and OVX-S, 21.9 ±4.0 ng/mL) compared to control (10±2.9 ng/mL) and OVX (2.8±0.5 ng/mL) groups. F active was higher in the OVX groups (OVX, 25.9±3.4 kN/m 2 and OVX-S, 26.3±3.0 kN/m2) than in control groups (control, 16.4±1.2 kN/m2 and control-S, 14.4±0.9 kN/m2). Regarding the potential molecular mechanisms, F active correlated with MyBP-C phosphorylation, while myofilament Ca2+ sensitivity inversely correlated with serum progesterone levels when the mean values were plotted for all animal groups. F passive was unaffected by any treatment.Conclusion Stress increases ovary- independent synthesis and release of progesterone, which may regulate Ca 2+ sensitivity of force production in left ventricular cardiomyocytes. Stress and female hormones differently alter Ca2+ dependent cardiomyocyte contractile force production, which may have pathophysiological importance during stress conditions affecting postmenopausal women

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Medicinski fakultet, Osijek,
Sveučilište u Osijeku - Odjel za biologiju

Profili:

Avatar Url Senka Blažetić (autor)

Avatar Url Marija Heffer (autor)

Avatar Url Marta Balog (autor)

Citiraj ovu publikaciju

Kalasz, Judit; Pasztor, Toth; Bodi, Beata; Fagyas, Miklos, Toth, Attila; Pal, Bgattoa H.; Vari Sandor G.; Balog, Marta; Blažetić, Senka; Heffer, Marija; Papp, Zoltan; Borbely, Attila
Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats // Croatian medical journal, 55 (2014), 3; 239-249 doi:10.3325/cmj.2014.55.239 (međunarodna recenzija, članak, znanstveni)
Kalasz, J., Pasztor, T., Bodi, B., Fagyas, Miklos, Toth, Attila, Pal, B., Vari Sandor G., Balog, M., Blažetić, S., Heffer, M., Papp, Z. & Borbely, A. (2014) Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats. Croatian medical journal, 55 (3), 239-249 doi:10.3325/cmj.2014.55.239.
@article{article, year = {2014}, pages = {239-249}, DOI = {10.3325/cmj.2014.55.239}, keywords = {Acute stress, ovariectomy, progesteron, cardiomyocytes, left ventriculi, rat}, journal = {Croatian medical journal}, doi = {10.3325/cmj.2014.55.239}, volume = {55}, number = {3}, issn = {0353-9504}, title = {Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats}, keyword = {Acute stress, ovariectomy, progesteron, cardiomyocytes, left ventriculi, rat} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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