engleski

Urinary metabolomic fingerprinting after consumption of a probioticstrain in women with mastitis

Infectious mastitis is a common condition among lactating women, with staphylococci and streptococcibeing the main aetiological agents. In this context, some lactobacilli strains isolated from breast milkappear to be particularly effective for treating mastitis and, therefore, constitute an attractive alternativeto antibiotherapy. A1H NMR-based metabolomic approach was applied to detect metabolomic differ-ences after consuming a probiotic strain (Lactobacillus salivarius PS2) in women with mastitis. Before24 h urine of women with lactational mastitis was collected at baseline and after 21 days of probiotic(PB) administration. Multivariate (OSC- PLS-DA and hierarchical clustering) analysis showed metabolomedifferences after PB treatment. The discriminant metabolites detected at baseline of the interventionwere lactose, and ibuprofen and acetaminophen (two pharmacological drugs commonly used for masti-tis pain), while, after PB intake, creatine and the gut microbial co- metabolites hippurate and TMAO weredetected. In addition, a voluntary desertion of the pharmacological drugs ibuprofen and acetaminophenwas observed after probiotic administration. The application of NMR- based metabolomics enabled theidentification of the overall effects of probiotic consumption among women suffering from mastitis andhighlighted the potential of this approach in evaluating the outcomes of probiotics consumption. To ourknowledge ; this is the first time that this approach has been applied in women with mastitis duringlactation.

Nutrimetabolomics; Metabolic biomarker; Mastitis; NMR; Probiotics; Lactobacillus salivarius

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