engleski

Toxicological Safety Assessment and Antioxidant Activity of Sodium Copper Chlorophyllin

Present study aimed to investigate the toxicological profile of chlorophyllin CHL) as well as its free radical scavenging capacity. Toxicological profile of CHL was assessed by measuring its cyto/genotoxic potential in human peripheral blood lymphocytes (HPBLs) after treatment with different concentrations (0.1-100 μg/ml) for 4 and 24 h. After the treatment, cytotoxicity and genotoxity was measured using a battery of bioassays. Results of cytotoxicity showed that CHL in tested concentrations had no effect on HPBLs viability. Additionally, CHL in concentration range tested did not cause DNA damage that could be detected with the comet assay and did not induce statistically significant difference in either micronucleus assay parameter. The antioxidant activity of CHL was determined using the DPPH assay. Results of DPPH assay revealed that CHL is an effective DPPH· scavenger with the concentration-dependent radical scavenging ability. The antioxidant activity of CHL when compared to some of well-known and powerful antioxidants, such as Trolox, ascorbic acid and butylated hydroxytoluene (BHT) was about 1.6 and 1.9 fold lower than that of ascorbic acid and Trolox, respectively, and 4.3 fold higher than that of BHT. Taken together, we have shown that CHL has no impact on cell viability and genome damage in the concentration range tested and therefore it can be considered safe from the aspect of cyto/genotoxicity. Since CHL also displayed radical scavenging ability it could be a perfect candidate for testing as a novel antioxidant.

chlorophyllin; human lymphocytes; cytotoxicity; genotoxicity; antioxidant activity

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