Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Characterizing cell migration in actin cytoskeleton mutants of Dictyostelium discoideum (CROSBI ID 611534)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Antolović, Vlatka ; Šmuc, Tomislav ; Weber, Igor. Characterizing cell migration in actin cytoskeleton mutants of Dictyostelium discoideum // The 14th International ELMI Meeting / Bakke, Oddmund ; Stenmark, Harald (ur.). Oslo, 2014. str. 133-134

Podaci o odgovornosti

Antolović, Vlatka ; Šmuc, Tomislav ; Weber, Igor.

engleski

Characterizing cell migration in actin cytoskeleton mutants of Dictyostelium discoideum

Cell motility is of the crucial importance for many physiological and pathological processes in multicellular organisms. The amoeboid protozoan Dictyostelium discoideum is a standard model organism used in cell motility research as well-suited for studying components of the actin cytoskeleton that are functionally conserved throughout the eukaryotic domain. Initial research of cell motility was based on the Brownian motion model describing random movement of small particles caused by thermal fluctuations. Over time, more precise measurements revealed intrinsic patterns in locomotion paths of eukaryotic cells, leading to modifications and extensions of the aforementioned model. The aim of this research is to characterize cell motility of actin cytoskeleton mutants of D. discoideum within the framework of a generalized Langevin model extended with a memory term. Furthermore, cell populations belonging to a single strain will be subdivided into subgroups based on motility characteristics of individual cells. D. discoideum cells grow as separate individual amoeba while in favorable condition, while upon starvation they drastically change their physiological state and interact with each other to form a multicellular slug. We monitored the motile behavior of two different strains of D. discoideum, wild-type strain (AX2), and the strain lacking cortexillins (CI-/CII-), in different stages of their life cycle: vegetative and aggregation-competent. Cortexillins are actin-bundling proteins and have an important role in the actin cytoskeleton organization. Cell movement was monitored by dark-field microscopy at low magnification. Images acquired at regular time intervals were stored and subsequently analyzed with the ParticleTracker plug-in for ImageJ. Results of the image processing are the cell tracks presented as time sequences of cell centroid coordinates. We identified different cell subpopulations by cluster analysis in RapidMiner environment using kmeans clustering algorithm with 14 parameters obtained by trajectory analysis. Parameters characterizing speed distribution were: the mean, the standard deviation, and the skewness and kurtosis of the distribution. Autocorrelograms of speed and velocity were characterized by their variance and the time lag of the first drop of an autocorrelogram below the threshold of significance. Periodograms of speed and velocity were characterized by their contributions in the following intervals: under a minute, between one and six minutes, and between six and 15 minutes. After the feature selection step, further clustering was performed using the two chosen parameters: the average speed and the sum of the periodogram values between time lags of 6 and 15 minutes. The later parameter represents a cell's oscillatory behavior in this range of time intervals. The starved cells of both AX2 and CI-/CII- strains were able to move faster than their vegetative counterparts, with the CI-/CII- cells showing a less strict control of speed in both phases of life cycle. CI-/CII- starved cells also display a loss of oscillatory behavior in the aggregation-competent phase.

cell motility; Dictyostelium discoideum; actin cytoskeleton

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

133-134.

2014.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

The 14th International ELMI Meeting

Bakke, Oddmund ; Stenmark, Harald

Oslo:

Podaci o skupu

The 14th International Advanced Light Microscopy Meeting (ELMI 2014)

poster

20.05.2014-23.05.2014

Oslo, Norveška

Povezanost rada

Biologija