Flow Injection Analysis of Antioxidants Capacity Based on Bienzymatic Production of ABTS•+ and Biamperometric Detector with Interdigitated Gold Electrodes (CROSBI ID 611500)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Milardović, Stjepan ; Kereković, Irena ; Rumenjak, Vlatko
engleski
Flow Injection Analysis of Antioxidants Capacity Based on Bienzymatic Production of ABTS•+ and Biamperometric Detector with Interdigitated Gold Electrodes
Development of flow injection analysis (FIA) is reported for determination of total antioxidant capacity of alcoholic beverages (wines and spirits) by bienzymatically produced ABTS+ and electrochemical detector using interdigitated gold electrode (IDE). The method is based on biamperometric measurements using ABTS+│ABTS redox couple. During the analysis, ABTS radical cation was bienzymatically produced by glucose- oxidase and peroxidase separately immobilized in tubular flow-through reactors. Influence of pH and substrate concentration on enzyme activity were tested in order to obtain the most sensitive mode. The effect of flow rate on operation of the bioreactors was also studied. Good comparison of results (R2 = 0.9942) was established for antioxidant capacity of tested samples between proposed FIA methods and reference spectrometric measurements.
antioxidant; ABTS; biamperometric detector
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Podaci o prilogu
2007.
objavljeno
Podaci o matičnoj publikaciji
XX hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka
Vrsalović Presečki, Ana ; Findrik, Zvjezdana
Zagreb:
Podaci o skupu
XX Hrvatski skup kemičara i kemijskih inženjera,
poster
26.02.2007-01.03.2007
Zagreb, Hrvatska