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Acetyl- and butyril- cholinesterase inhibitory activity of Lamiaceae plant extracts


Generalić Mekinić, Ivana; Burčul, Franko; Skroza, Danijela; Miloš, Mladen; Katalinić, Višnja
Acetyl- and butyril- cholinesterase inhibitory activity of Lamiaceae plant extracts // 7th Central European Congress on Food, Book of Abstracts / Kakurinov, Vladimir (ur.).
Skopje: Consulting and Training Center KEY, 2014. str. 85-85 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Acetyl- and butyril- cholinesterase inhibitory activity of Lamiaceae plant extracts

Autori
Generalić Mekinić, Ivana ; Burčul, Franko ; Skroza, Danijela ; Miloš, Mladen ; Katalinić, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Central European Congress on Food, Book of Abstracts / Kakurinov, Vladimir - Skopje : Consulting and Training Center KEY, 2014, 85-85

ISBN
978-608-4565-05-5

Skup
7th Central European Congress on Food

Mjesto i datum
Ohrid, Makedonija, 21-24.05.2014.

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Acetylcholine; Acetylcholinesterase; Butyrilcholinesterase; Inhibitory activity; Lamiaceae

Sažetak
Cholinesterase is a family of enzymes that catalyze the hydrolysis of acetylcholine into choline and acetic acid. Acetylcholine is a neurotransmitter inhibited primarily by acetylcholinesterase (AChE) and secondly by butyrylcholinesterase (BuChE). The pathogenesis of Alzheimer’s disease, a progressive degenerative neurological disorder and the most common form of dementia, has been linked to a deficiency of this neurotransmitter in brain. Subsequently, AChE and BuChE inhibitors were introduced for the symptomatic treatment of this disease. They inhibit the enzymes from breaking down acetylcholine, thereby increasing both the level and duration of the action of the neurotransmitter acetylcholine. Apart the common synthetic drugs, variety of phytochemicals and plant extracts have been reported to show cholinesterase inhibitory activity. The aim of this study was to explore possible AChE or BuChE inhibitors in plants from the Lamiaceae family, namely sage, thyme, lemon balm, mint and oregano. Plant materials were purchased from a local herbal pharmacy. Homogenized material (5 g) was extracted with 80% ethanol (250 mL) at 60°C for 60 min. The extractions were performed in triplicate and after the filtration extracts were combined and evaporated to the volume of 150 mL. The prepared extracts were used in AChE/BuChe inhibitory activity measurements by slightly modified Ellman method. According to the obtained results, all extracts posses AChE and BuChE inhibitory activity. The best results for AChE were obtained for thyme and sage extracts (63 and 61% respectively), while in addition, the sage extract was extremely effective against BuChE (81%). The lowest activity on both enzymes was detected for lemon balm and oregano extracts. The results of this investigation open new possibilities of using these well-known medicinal plants in pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija



POVEZANOST RADA


Projekt / tema
011-2160547-1330 - Antioksidacijski sastojci i inhibitori kolinesteraza iz aromatičnog bilja (Mladen Miloš, )
011-2160547-2226 - Prirodni izvori resveratrola i njegov sinergijski učinak s drugim polifenolima (Višnja Katalinić, )

Ustanove
Kemijsko-tehnološki fakultet, Split