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Pregled bibliografske jedinice broj: 697868

Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid beta1-42 and total tau


Babić, Mirjana; Vogrinc, Željka; Diana, Andrea; Klepac, Nataša; Borovečki, Fran; Hof, P.R.; Šimić, Goran
Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid beta1-42 and total tau // Translational neuroscience, 4 (2013), 2; 234-240 doi:10.2478/s13380-013-0123-4 (međunarodna recenzija, članak, znanstveni)


Naslov
Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid beta1-42 and total tau

Autori
Babić, Mirjana ; Vogrinc, Željka ; Diana, Andrea ; Klepac, Nataša ; Borovečki, Fran ; Hof, P.R. ; Šimić, Goran

Izvornik
Translational neuroscience (2081-3856) 4 (2013), 2; 234-240

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Alzheimer’s disease; biomarkers; cerebrospinal fluid

Sažetak
Amyloid β1–42 (Aβ1–42), total tau (t-tau), and phosphorylated tau (p-tau) are the main cerebrospinal fluid (CSF) biomarkers for early diagnosis of Alzheimer’s disease (AD). Detection of AD is critically important in view of the growing number of potential new drugs that may influence the course of the disease in its early phases. However, cut-off levels for these CSF biomarkers have not yet been established. Variability in absolute concentrations of AD biomarkers is high among studies and significant differences were noticed even within the same datasets. Variability in biomarkers levels in these assays may be due to many aspects of operating procedures. Standardization of pre- analytical and analytical procedures in collection, treatment, and storage of CSF samples is crucial because differences in sample handling can drastically influence results. Multicenter studies showed that usage of ELISA kits from different manufacturers also affects outcome. So far only very few studies tested the efficiency of ELISA kits produced by different vendors. In this study, the performance of Innogenetics (Gent, Belgium) and Invitrogen (Camarillo, CA, USA) ELISA kits for t-tau and Aβ1–42 was tested. Passing- Bablok analysis showed significant differences between Invitrogen and Innogenetics ELISA methods, making it impossible to use them interchangeably.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081870-1942 - Fosforilacija tau proteina u razvitku i Alzheimerovoj bolesti (Goran Šimić, )
HRZZ-09/16 - Otkrivanje i praćenje bioloških biljega radi rane terapijske intervencije u Alzheimerovoj bolesti (Goran Šimić, )
IP-2014-09-4503

Ustanove
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • Journal Citation Reports/Science Edition, SCOPUS, EMBASE, Google Scholar, BIOSIS, OCLC, SCImago, Summon by ProQuest


Citati