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Lymphocyte and monocyte research population data by UniCel® DxH 800 Coulter® in blood samples of HIV-infected patients

Introduction: Hematologic manifestations of HIV infection usually include lymphocytopenia, thrombocytopenia and anemia. As HIV infects CD4+ cells, we decided to evaluate the Unicel® DxH 800 Hematology analyzer performances to analyze research population data (RPD) for lymphocytes and monocytes in HIV-infected patients. RPD on Unicel® DxH 800 enable detailed analysis of leukocytes differential based on the measurements of: volume (V), conductivity (C) and five different angles of Scatter light laser (Medium angle light scatter-MALS, Low medium angle light scatter-LMALS, Upper medium angle light scatter-UMALS, Axial light loss-ALL, Low angle light scatter-LALS). Methods: We analyzed RPD for lymphocytes and monocytes in blood samples from 32 HIV-infected patients who had complete blood count (CBC) parameters within reference range (except higher MCV values in some patients). K3-EDTA blood samples were collected from HIV-infected patients during their regular visit in Outpatient Department for HIV infection. They were compared with control blood samples taken from 39 healthy workers from the same Hospital. Results: Results for mean (SD) or median (95% CI for the median) and p values for HIV-infected patients and controls were as follows: Ly-V: 89 (88, 00-90, 00) vs 85 (83, 10-86, 79), p<0, 0001 ; Ly-C: 117, 50 (117, 00-118, 79) vs 120 (120, 00-120, 00), p<0, 0001 ; Ly-MALS: 66, 53 (4, 09) vs 61, 82 (4, 04), p<0, 0001 Ly-LMALS: 62, 56 (3, 86) vs 58, 13 (2, 13), p<0, 0001 Ly-UMALS: 62, 97 (4, 95) vs 57, 33 (7, 28), p=0, 0004 Ly-ALL: 65, 00 (64, 00-65, 00) vs 70, 00 (69, 00-71, 00), p<0, 0001 Ly-LALS: 42, 00 (41, 00-42, 00) vs 42, 00 (42, 00-43, 00), p=0, 1215 Mo-V: 172, 00 (170, 00-176, 79) vs 170, 00 (168, 00-171, 00), p=0, 0202 ; Mo-C: 123, 53 (2, 08) vs 126, 44 (1, 85), p<0, 0001 ; Mo-MALS: 86, 25 (2, 20) vs 81, 05 (2, 21), p<0, 0001 Mo-LMALS: 73, 19 (2, 96) vs 66, 31 (2, 77), p<0, 0001 Mo-UMALS: 94, 72 (3, 09) vs 91, 72 (2, 73), p<0, 0001 Mo-ALL: 110, 94 (5, 98) vs 119, 79 (4, 75), p<0, 0001 Mo-LALS: 83, 72 (14, 47) vs 80, 95 (13, 14), p=0, 4013 Conclusion: RPD have been used lately in early detection of sepsis, malaria and some hematological disorders. Most of RPD results for lymphocytes and monocytes in blood samples of HIV-infected patients were different from the control group, although their CBC parameters were within limits. These findings should be evaluated on a larger sample size taking into account HIV disease parameters (CD4 cell count, viral load, clinical AIDS) and type of antiretroviral treatment.

HIV; Research population data; Unicel DxH 800 Coulter

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