engleski

Serotype Distribution of Chlamydia trachomatis Isolated from clinical urogenital samples in North-Eastern Croatia

The fast molecular diagnostics of Chlamydia trachomatis infections and adequate therapy of the infected individuals are the crucial step in the C. trachomatis spread control. Due to the chronic and "silent" infection and variable antigenic structure of the C.trachomatis we supplemented Direct Immune Fluorescence with the molecular method RT-PCR. The aim of the present study has been to reveal the most prevalent serotype of the C. trachomatis detected in urine and gynaecological samples and to monitor the infection and the therapy efficiency. The determined serotype distribution has been compared with the C. trachomatis distribution pattern in other regions of the World. COBAS TaqMan CT test is an in vitro nucleic acid amplification test which utilizes real time PCR technology. The test has been developed to confirm analogous detection of all 15 C. trachomatis serotypes. Subsequently all the positive samples have been analysed directly by sequencing of the amplified omp1 fragments using Applied Biosystems 3130 Genetic Analyser. Genotyping and sequence mutation analysis have been performed using ABI SEQSCAPE software and compared with the reference sequences of all known C. trachomatis serotypes. The preliminary study determined serotype E (in agreement with Sweden and Taiwan data) as the dominant one, followed by F, K, G, D, B, J and Ia (differs Sweden and Taiwan data). Further investigation and data analysis are in progress.

urogenital infections ; C. trachomatis diagnostics ; serotypes ; sequencing

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