engleski

Genotyping of Human Papillomavirus in abnormal cervical samples from the North-Eastern Croatia

Human papillomavirus (HPV) is one of the most common sexually transmitted diseases worldwide. Most HPV infections are cleared by the immune system and do not result in clinical complications. Clinical consequences in case of low-risk HPV infection consist of genital warts and mild Pap test abnormalities, while High-risk HPV infection (HR HPV), especially with oncogenic HPV types HPV 16 and HPV 18, include abnormal Pap results, low and high-grade cervical intraepithelial neoplasia and cervical cancer, for which Chlamydia trachomatis may sometimes be a co- factor. During three-year period we tested 1194 clinical specimens for HR HPV ; 249 urethral swabs from men who had sexual contact with an infected person and 945 cervical swabs with normal and abnormal cervical cytological diagnosis. All samples were collected in the Osijek-Baranja County Institute of Public Health and gynaecologist's offices. Subsequently 100 HR HPV positive cervical samples with abnormal cervical cytological diagnosis were genotyped by Linear Array HPV Genotyping Test (Roche Diagnostics). The association between certain HPV genotype and cervical intraepithelial neoplasia was determined as well as mixed infections with four or more HPVs. The aim of the study was to determine the prevalence of HR HPV and age-related profiles of HR HPV infections in population of our county. The obtained data indicate that HPV 16 and HPV 18 are the most common HR HPV genotypes in the cervical specimens with abnormal cervical cytological diagnosis among women in Osijek-Baranja County as in most studies worldwide. Obtained results also determined mixed infection of four HR HPV with one or more low risk HPV. Our results revealed that cervical HR HPV infection is strongly related to age, with highest occurrence at the age 25-34, in association with increased number of sexual partners. The obtained results and further analyses in this ongoing project could be useful tool for clinical and epidemiological characterization of HPV in our community.

sexually transmitted diseases; high-risk HPV; low-risk HPV; HPV genotype

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