engleski

Laccase Inhibiting Activity of Some Coumarin Derivatives

Seven coumarin derivatives were screened for their inhibitory effect on laccase utilizing ABTS and L- DOPA as substrates. 2-[(4-Methyl-2-oxo-2H-1- benzopyran-7-yl)oxy]acetic acid (E)-2- [(dihydroxyphenyl)methylene]hydrazides were proven to be stronger inhibitors than corresponding thiosemicarbazides when ABTS [2, 2'-azinobis(3- ethylbenzothiazoline-6-sulfonic acid)] was used as a substrate. In kinetic experiments mixed type inhibition was determined for 2-[(4-methyl-2-oxo- 2H-1-benzopyran-7-yl)oxy]acetic acid (E)-2-[(2, 5- dihydroxyphenyl)methylene]hydrazide and (E)-2- [(3, 4-dihydroxyphenyl)methylene]hydrazide. In reaction of L-DOPA (L-3, 4-dihydroxyphenylalanine) oxidation catalyzed by laccase none of the tested compounds has shown inhibitory effect.

coumarins; laccase activity; inhibition; ABTS; L-DOPA.

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