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Pregled bibliografske jedinice broj: 696105

Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA


Simunić, Juraj; Petrov, Dražen; Bouceba, Tahar; Kamech, Nédia; Benincasa, Monica; Juretić, Davor
Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA // Biochimica et biophysica acta. Biomembranes, 1838 (2014), 5; 1430-1438 doi:10.1016/j.bbamem.2014.02.003 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 696105 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA

Autori
Simunić, Juraj ; Petrov, Dražen ; Bouceba, Tahar ; Kamech, Nédia ; Benincasa, Monica ; Juretić, Davor

Izvornik
Biochimica et biophysica acta. Biomembranes (0005-2736) 1838 (2014), 5; 1430-1438

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antimicrobial peptide; EST database; Trichoplax adhaerens; selectivity index; surface plasmon resonance; peptide-membrane interaction

Sažetak
A method based on the use of signal peptide sequences from antimicrobial peptide (AMP) precursors was used to mine a placozoa expressed sequence tag database and identified a potential antimicrobial peptide from Trichoplax adhaerens. This peptide, with predicted sequence FFGRLKSVWSAVKHGWKAAKSR is the first AMP from a placozoan species, and was named trichoplaxin. It was chemically synthesized and its structural properties, biological activities and membrane selectivity were investigated. It adopts an α- helical structure in contact with membrane-like environments and is active against both Gram- negative and Gram-positive bacterial species (including MRSA), as well as yeasts from the Candida genus. The cytotoxic activity, as assessed by the haemolytic activity against rat erythrocytes, U937 cell permeabilization to propidium iodide and MCF7 cell mitochondrial activity, is significantly lower than the antimicrobial activity. In tests with membrane models, trichoplaxin shows high affinity for anionic prokaryote-like membranes with good fit in kinetic studies. Conversely, there is a low affinity for neutral eukaryote-like membranes and absence of a dose dependent response. With high selectivity for bacterial cells and no homologous sequence in the UniProt, trichoplaxin is a new potential lead compound for development of broad- spectrum antibacterial drugs.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

Napomena
Highlights • First report of antimicrobial peptide from placozoa EST database • Broad activity spectrum against G − and G + bacteria, including MRSA • Low toxicity and high selectivity for prokaryotic- like membranes.



POVEZANOST RADA


Projekti:
177-1770495-0476 - Razvoj i primjene principa maksimalne proizvodnje entropije (Juretić, Davor, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Split

Profili:

Avatar Url Davor Juretić (autor)

Avatar Url Dražen Petrov (autor)

Avatar Url Juraj Simunić (autor)

Citiraj ovu publikaciju

Simunić, Juraj; Petrov, Dražen; Bouceba, Tahar; Kamech, Nédia; Benincasa, Monica; Juretić, Davor
Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA // Biochimica et biophysica acta. Biomembranes, 1838 (2014), 5; 1430-1438 doi:10.1016/j.bbamem.2014.02.003 (međunarodna recenzija, članak, znanstveni)
Simunić, J., Petrov, D., Bouceba, T., Kamech, N., Benincasa, M. & Juretić, D. (2014) Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA. Biochimica et biophysica acta. Biomembranes, 1838 (5), 1430-1438 doi:10.1016/j.bbamem.2014.02.003.
@article{article, year = {2014}, pages = {1430-1438}, DOI = {10.1016/j.bbamem.2014.02.003}, keywords = {antimicrobial peptide, EST database, Trichoplax adhaerens, selectivity index, surface plasmon resonance, peptide-membrane interaction}, journal = {Biochimica et biophysica acta. Biomembranes}, doi = {10.1016/j.bbamem.2014.02.003}, volume = {1838}, number = {5}, issn = {0005-2736}, title = {Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA}, keyword = {antimicrobial peptide, EST database, Trichoplax adhaerens, selectivity index, surface plasmon resonance, peptide-membrane interaction} }
@article{article, year = {2014}, pages = {1430-1438}, DOI = {10.1016/j.bbamem.2014.02.003}, keywords = {antimicrobial peptide, EST database, Trichoplax adhaerens, selectivity index, surface plasmon resonance, peptide-membrane interaction}, journal = {Biochimica et biophysica acta. Biomembranes}, doi = {10.1016/j.bbamem.2014.02.003}, volume = {1838}, number = {5}, issn = {0005-2736}, title = {Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA}, keyword = {antimicrobial peptide, EST database, Trichoplax adhaerens, selectivity index, surface plasmon resonance, peptide-membrane interaction} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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