PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies (CROSBI ID 205601)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Milovac, Dajana ; Gamboa-Martínez, Tatiana C. ; Ivanković, Marica ; Gallego Ferrer, Gloria ; Ivanković, Hrvoje
engleski
PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies
In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)- coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope.
cuttlefish bone; poly(ε-caprolactone); hydroxyapatite scaffold; tissue engineering; cell differentiation
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Podaci o izdanju
42
2014.
264-272
objavljeno
0928-4931
10.1016/j.msec.2014.05.034
Povezanost rada
Kemija, Kemijsko inženjerstvo