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Pregled bibliografske jedinice broj: 696096

PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies


Milovac, Dajana; Gamboa-Martínez, Tatiana C.; Ivanković, Marica; Gallego Ferrer, Gloria; Ivanković, Hrvoje
PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies // Materials Science and Engineering C. Materials for Biological Applications, 42 (2014), 264-272 doi:10.1016/j.msec.2014.05.034 (međunarodna recenzija, članak, znanstveni)


Naslov
PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies

Autori
Milovac, Dajana ; Gamboa-Martínez, Tatiana C. ; Ivanković, Marica ; Gallego Ferrer, Gloria ; Ivanković, Hrvoje

Izvornik
Materials Science and Engineering C. Materials for Biological Applications (0928-4931) 42 (2014); 264-272

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cuttlefish bone; poly(ε-caprolactone); hydroxyapatite scaffold; tissue engineering; cell differentiation

Sažetak
In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)- coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo



POVEZANOST RADA


Projekt / tema
125-1252970-3005 - Biokeramički, polimerni i kompozitni nanostrukturirani materijali (Hrvoje Ivanković, )

Ustanove
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Hrvoje Ivanković (autor)

Avatar Url Marica Ivanković (autor)

Avatar Url Dajana Milovac (autor)

Citiraj ovu publikaciju

Milovac, Dajana; Gamboa-Martínez, Tatiana C.; Ivanković, Marica; Gallego Ferrer, Gloria; Ivanković, Hrvoje
PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies // Materials Science and Engineering C. Materials for Biological Applications, 42 (2014), 264-272 doi:10.1016/j.msec.2014.05.034 (međunarodna recenzija, članak, znanstveni)
Milovac, D., Gamboa-Martínez, T., Ivanković, M., Gallego Ferrer, G. & Ivanković, H. (2014) PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies. Materials Science and Engineering C. Materials for Biological Applications, 42, 264-272 doi:10.1016/j.msec.2014.05.034.
@article{article, year = {2014}, pages = {264-272}, DOI = {10.1016/j.msec.2014.05.034}, keywords = {cuttlefish bone, poly(ε-caprolactone), hydroxyapatite scaffold, tissue engineering, cell differentiation}, journal = {Materials Science and Engineering C. Materials for Biological Applications}, doi = {10.1016/j.msec.2014.05.034}, volume = {42}, issn = {0928-4931}, title = {PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies}, keyword = {cuttlefish bone, poly(ε-caprolactone), hydroxyapatite scaffold, tissue engineering, cell differentiation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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