engleski

Laminar and Temporal Expression Dynamics of Coding and Noncoding RNAs in the Mouse Neocortex

The hallmark of the cerebral neocortex is its organization into six layers, each containing a characteristic set of cell types and synaptic connections. The transcriptional events involved in laminar development and function still remain elusive. Here we employed deep sequencing of mRNA and small RNA species to gain insights into transcriptional differences among layers and their temporal dynamics during postnatal development of the mouse primary somatosensory neocortex. A number of novel coding and noncoding transcripts were identified with specific spatiotemporal expression and splicing patterns. We also identified signature trajectories and gene coexpression networks associated with distinct biological processes and transcriptional overlap between these processes. Finally, we provide data that allows the study of potential miRNA and mRNA interactions. Overall, this study provides an integrated view of the laminar and temporal expression dynamics of coding and noncoding transcripts in the mouse neocortex and a resource for studies of neurodevelopment and transcriptome.

RNA-Seq; Transcriptome; mouse neocortex; small RNA; development; non-coding RNA; coding RNA; spatiotemporal gene expression; neocortical layers; developmental trajectory; miRNA-mRNA regulation

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