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NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans


Fanczalszky, Luca; Monostori, Eszter; Farkas, Zsolt, Pourkarimi, Ahsan; Masoudi, Neda; Hargitai, Balazs; Herak Bosnar, Maja; Deželjin, Martina; Zsakai, Annamaria; Vellai Tibor; Mehta, Anil et al.
NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans // PLoS One, 9 (2014), 3; e92687-e92687 doi:10.1371/journal.pone.0092687 (međunarodna recenzija, članak, znanstveni)


Naslov
NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans

Autori
Fanczalszky, Luca ; Monostori, Eszter ; Farkas, Zsolt, Pourkarimi, Ahsan ; Masoudi, Neda ; Hargitai, Balazs ; Herak Bosnar, Maja ; Deželjin, Martina ; Zsakai, Annamaria ; Vellai Tibor ; Mehta, Anil ; Mehta, Anil ; Takacz-Vellai, Krisztina

Izvornik
PLoS One (1932-6203) 9 (2014), 3; E92687-e92687

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NDK ; nm23 ; C. elegans ; apoptotic engulfment

Sažetak
Abnormal regulation of cell migration and altered rearrangement of cytoskeleton are characteristic of metastatic cells. The first described suppressor of metastatic processes is NM23-H1, which displays NDPK (nucleoside-diphosphate kinase) activity. To better understand the role of nm23 genes in cell migration, we investigated the function of NDK-1, the sole Caenorhabditis elegans homolog of group I NDPKs in distal tip cell (DTC) migration. Dorsal phase of DTC migration is regulated by integrin mediated signaling. We find that ndk-1 loss of function mutants show defects in this phase. Epistasis analysis using mutants of the α- integrin ina-1 and the downstream functioning motility-promoting signaling module (referred to as CED-10 pathway) placed NDK-1 downstream of CED-10/Rac. As DTC migration and engulfment of apoptotic corpses are analogous processes, both partially regulated by the CED-10 pathway, we investigated defects of apoptosis in ndk-1 mutants. Embryos and germ cells defective for NDK-1 showed an accumulation of apoptotic cell corpses. Furthermore, NDK-1::GFP is expressed in gonadal sheath cells, specialized cells for engulfment and clearence of apoptotic corpses in germ line, which indicates a role for NDK-1 in apoptotic corpse removal. In addition to the CED-10 pathway, engulfment in the worm is also mediated by the CED-1 pathway. abl-1/Abl and abi-1/Abi, which function in parallel to both CED-10/CED-1 pathways, also regulate engulfment and DTC migration. ndk-1(-) ; abi-1(-) double mutant embryos display an additive phenotype (e. g. enhanced number of apoptotic corpses) which suggests that ndk-1 acts in parallel to abi-1. Corpse number in ndk-1(-) ; ced- 10(-) double mutants, however, is similar to ced-10(-) single mutants, suggesting that ndk-1 acts downstream of ced-10 during engulfment. In addition, NDK-1 shows a genetic interaction with DYN- 1/dynamin, a downstream component of the CED-1 pathway. In summary, we propose that NDK-1/NDPK might represent a converging point of CED-10 and CED-1 pathways in the process of cytoskeleton rearrangement.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2513 - Uloga gena nm23 u karcinomu pločastog epitela usne šupljine (Maja Herak-Bosnar, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Fanczalszky, Luca; Monostori, Eszter; Farkas, Zsolt, Pourkarimi, Ahsan; Masoudi, Neda; Hargitai, Balazs; Herak Bosnar, Maja; Deželjin, Martina; Zsakai, Annamaria; Vellai Tibor; Mehta, Anil et al.
NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans // PLoS One, 9 (2014), 3; e92687-e92687 doi:10.1371/journal.pone.0092687 (međunarodna recenzija, članak, znanstveni)
Fanczalszky, L., Monostori, E., Farkas, Zsolt, Pourkarimi, Ahsan, Masoudi, N., Hargitai, B., Herak Bosnar, M., Deželjin, M., Zsakai, A., Vellai Tibor & Mehta, A. (2014) NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans. PLoS One, 9 (3), e92687-e92687 doi:10.1371/journal.pone.0092687.
@article{article, year = {2014}, pages = {e92687-e92687}, DOI = {10.1371/journal.pone.0092687}, keywords = {NDK, nm23, C. elegans, apoptotic engulfment}, journal = {PLoS One}, doi = {10.1371/journal.pone.0092687}, volume = {9}, number = {3}, issn = {1932-6203}, title = {NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans}, keyword = {NDK, nm23, C. elegans, apoptotic engulfment} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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