Nephroprotective effect of alkaloid berberine against cisplatin-induced kidney damage in mice (CROSBI ID 610071)
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Podaci o odgovornosti
Domitrović, Robert ; Gobin, Ivana ; Cvijanović, Olga ; Pernjak-Pugel, Ester ; Škoda, Marko ; Mikelić, Lorena
engleski
Nephroprotective effect of alkaloid berberine against cisplatin-induced kidney damage in mice
The aim of this study was to investigate the therapeutic activity of isoquinoline alkaloid berberine against cisplatin (CP)-induced nephrotoxicity in mice. Berberine was administered at daily doses of 1, 2 and 3 mg/kg by gavage for two succesive days, 48 h after intraperitoneal CP injection (13 mg/kg). Mice were sacrificed 24 h after the last dose of berberine. Histopathological changes and the increase in serum creatinine and blood urea nitrogen (BUN) induced by CP were significantly ameliorated by berberine in a dose-dependent manner. Additionally, oxidative/nitrosative stress, evidenced by the increase in renal 4- hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), cytochrome P450 E1 (CYP2E1) and heme oxygenase (HO-1) expression, was significantly reduced. The expression of nuclear factor-kappaB (NF-κB), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX- 2) and inducible nitric oxide synthase (iNOS) was markedly suppressed by berberine, indicating the inhibition of inflammatory response. Treatment of CP-intoxicated animals with berberine also significantly reduced the expression of p53, active caspase-3 as well as autophagy marker light chain 3B (LC3B) in the kidneys. The results of the current study show the nephroprotective activity of berberine against CP-induced renal injury, which could be attributed to the inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis.
berberine; cisplatin nephrotoxicity; oxidative stress; inflammatory response; autophagy; apoptosis
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Podaci o prilogu
66-66.
2014.
objavljeno
Podaci o matičnoj publikaciji
Abstracts
Dubai:
Podaci o skupu
3rd Biotechnology World Congress
poster
10.02.2014-12.02.2014
Dubai, Ujedinjeni Arapski Emirati