engleski

The impared recruitment and functional maturation of CD4+ T-cells in chronic lymphocytic leukemia (CLL) patients

Chronic lymphocytic leukemia (CLL) ia a lymphoproliferative disorder characterized by clonal expansion of a small long-lived immature CD5+ B-lymphocytes. The imbalanced and/or functionally alered T-cell population or some of its subsets could be of importance in CLL pathogenesis. CD4+ T-cell express multiple CD45 molecule isoforms that are specific and changeable depending on their differentiation and activation state. In this study we investigated the frequency of CD4+ cells and their naive (CD45RA+) intermediate (CD45RA+RO+) or memory (CD45RO+) subsets in the peripheral blood of CLL patients. In order to detect simultaneously the expression of CD45RA+ and CD45RO+ antigens on CD4+ T-cells, we used 3-color flow cytometry immunofluorescence analysis of the whole blood. Heparinized peripheral blood samples obtained from 48 CLL patients classified according to the modified Rai staging system into low, intermediate and high risk groups and from 19 age and gender matched healthy subjects were used. We found the percentage of double positive CD4+CD45RO+RA+ T-cells to be significantly increased in low (p<0.05), intermediate (p<0.01) and high (p<0.01) risk CLL patients when compared to healthy subjects. Moreover, the percentage of CD45RO+RA+ CD4+ T-cells positively correlates with modified Rai staging system (r00.49 p<0.001). In addition the significan decreased percentage of CD45RA+ CD4+ T-cells(p<0.01) in the high risk CLL groups was found. Finding that in CLL patients along with decreased percentage of naive T-cells in the high risk group, and increased the percentage of double positive CD45RO+RA+ CD4+ t-cells might reflect a progressive imairment of their recruitment and inability to maturate functionaly and adequately participate in the control of B-cell malignant clone expansion.

chronic lymphocytic leukemia; T-cells; CD4+ cells; CD45RA; CD45RO; disease stage

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