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Pregled bibliografske jedinice broj: 692044

Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics


Krilov, Dubravka; Kosović, Marin; Serec, Kristina
Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics // Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 129 (2014), 588-593 doi:10.1016/j.saa.2014.03.087 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 692044 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics

Autori
Krilov, Dubravka ; Kosović, Marin ; Serec, Kristina

Izvornik
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (1386-1425) 129 (2014); 588-593

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
liposome; α – tocopherol; lipid layer; oxidation; FT-IR; EPR

Sažetak
The influence of α – tocopherol on the surface conformation of liposome, as a model component of lipoproteins, and its role in oxidation process were studied. FT-IR spectra from suspensions of neat liposome, mixtures of liposome and α – tocopherol and liposome with incorporated α – tocopherol were analyzed. When α – tocopherol was incorporated into liposome, intensities of some bands were decreased or increased in comparison with the spectra of liposome and α – tocopherol mixture. These changes reflect the different localization of α – tocopherol in two types of liposome suspensions. The oxidation of liposome suspensions was initiated by addition of cupric ions. After prolonged oxidation, the differences in FT-IR spectra of oxidized samples were recorded. Differences were observed in comparison with spectra of native and oxidized liposomes were analyzed. The rate of oxidation was measured by EPR oximetry. Oxidation was generally very slow, but faster in liposome without α – tocopherol, indicating the protective role of α – tocopherol against liposome oxidation. On the other hand, liposome suspensions with EDTA in the buffer were not oxidized at all, while those with α – tocopherol and liposome mixture were only slightly oxidized. In this case the consumption of oxygen was the result of liposome oxidation supported by α – tocopherol. These results reflect the ambivalent role of α – tocopherol in liposome oxidation, similarly to findings in studies of lipoprotein oxidation.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Projekti:
108-1080134-3105 - Mehanizmi narušavanja strukture lipoproteina djelovanjem vanjskih čimbenika (Gamulin, Ozren, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Dubravka Krilov (autor)

Avatar Url Marin Kosović (autor)

Avatar Url Kristina Serec (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Krilov, Dubravka; Kosović, Marin; Serec, Kristina
Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics // Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 129 (2014), 588-593 doi:10.1016/j.saa.2014.03.087 (međunarodna recenzija, članak, znanstveni)
Krilov, D., Kosović, M. & Serec, K. (2014) Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 129, 588-593 doi:10.1016/j.saa.2014.03.087.
@article{article, year = {2014}, pages = {588-593}, DOI = {10.1016/j.saa.2014.03.087}, keywords = {liposome, α – tocopherol, lipid layer, oxidation, FT-IR, EPR}, journal = {Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy}, doi = {10.1016/j.saa.2014.03.087}, volume = {129}, issn = {1386-1425}, title = {Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics}, keyword = {liposome, α – tocopherol, lipid layer, oxidation, FT-IR, EPR} }
@article{article, year = {2014}, pages = {588-593}, DOI = {10.1016/j.saa.2014.03.087}, keywords = {liposome, α – tocopherol, lipid layer, oxidation, FT-IR, EPR}, journal = {Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy}, doi = {10.1016/j.saa.2014.03.087}, volume = {129}, issn = {1386-1425}, title = {Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics}, keyword = {liposome, α – tocopherol, lipid layer, oxidation, FT-IR, EPR} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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