engleski

Peripheral expression of adipocytokines and role of sympathetic nervous system in function of perivascular adipose tissue and vascular reactivity

Aim: Visceral (VAT) and perivascular adipos e tissue (PAT) are ac tive endocrine and paracrine organs. Two opposite functions are assigned to PAT. It has antiatherogenic and proatherogenic effects. In this research, ch anges of PAT and sympathetic nervous system will be measured also influence of mother `s nutrition during gest ation on function of PAT and VAT in offspri ng will be determined. Methods: 10 female rats will be ra ndomly divided in 2 groups. One gr oup will be fed with high content of saturate d fatty acid – FD group (n=5), and the other will be fed with standard laboratory chow – CD group (n=5). After coupling and lactation offspring will be randomly divided in 2 groups – one will be fed with high content of saturated fatty acid (FD-FD2 and CD- FD2), and other with st andard laboratory chow (FD-CD2 and CD- CD2). We will have 4 groups of offspring ge netically similar, but exposed to different intrauterine and postnatal nutritional envi ronments. Also, blood concentrations of adiponectin, insulin, glucose, LD L, HDL, triglycerides, TNF- α and IL-6 in four separate groups of animals will be measured. After decapitation, expression of ADIPOQ and β 3- AR gene will be measured in samples of VAT and PAT, and expression of SLC6A2 and D β H will be measured in coeliac ganglion. Ro le of PAT will be tested by measuring vascular reactivity after norepinephrine perfus ion through isolated br anches of superior mesenteric artery. Results: By measuring expression of ADIPOQ gene and β 3-AR gene in adipose cells of PAT in regard to different environment of o ffspring we expect to confirm that lipolysis mediated by sympathetic nervous system in pr edisposed rats is decreased. By analyzing offspring of fat diet mothers which are on control diet we w ill attempt to determine role of postnatal diet on sympathetic nervous system activity in PAT and synthesis of adiponectin in PAT a nd VAT. We will try to link the changes in ADIPOQ gene expression in PAT with ch anges in expression of SLC6A2 gene and D β H gene in coeliac ganglion . Also, we expect to comfir m protective role of PAT in control diet rats and lack of that protection in fat diet rats by measuri ng vascular reactivity of superior mesenteric artery with and without PAT. Conclusions: Understanding relationship between PA T and sympathetic nervous system will enable us to create mo re rational pharmacotherapy for obesity and metabolic syndrome. In general, it could enable grea t social, economic and health benefits for individuals and society.

visceral adipose tissue; perivascular adipose tissue; sympathetic nervous system

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