Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces (CROSBI ID 203579)
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Podaci o odgovornosti
Penezić, Abra ; Deokar, Geetanjali ; Vignaud, Dominique ; Pichonat, Emmanuelle ; Subramanian, Palaniappan ; Gašparović, Blaženka ; Boukherroub, Rabah ; Szunerits, Sabine
engleski
Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces
The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapour deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single and double layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent -stacking interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum Vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.
graphene; surface plasmon resonance; mannose; lectins; biosensors
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