engleski

Axillary shoot proliferation and in vitro rooting of apple cv. Topaz - impacts of treatments and explant orientation

Topaz belongs to a group of apple varieties with high sugar content and high acidity, developed in Czech Republic. Besides its fruit quality preferred by consumers, ´Topaz´ is also scab-resistant. This fact is the most important characteristic which is providing another reason for significant spread of this cultivar in European orchards, especially in the organic fruit production industry. Considering the sparse information about the potential of this apple tree to be propagated in vitro, the role of several factors on micropropagation of apple cv. Topaz was evaluated. The results showed that 1 mg/L thidiazuron (TDZ) gave the highest number of shoots (3.6) per inoculated shoot, but the shoots obtained on this medium were fasciated. Media with 0.5 or 1 mg/L 6-benzylaminopurine (BA) or BA (0.5) + 1.5 mg/L kinetin (Kin) produced in average of 1.9-2.5 microshoots with no significant differences among them. Considering different explants types the best results gave decapitated microshoots placed vertically although not significantly different from those obtained from intact microshoots placed vertically and one-nodal segments placed horizontally. Shoots treated with 2 mg/L of indole-3-butyric acid (IBA) showed the highest average root number (6.6) and the highest percentage of rooting (68.7%).

apple; Topaz; micropropagation; media; explant type; rooting

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