Napredna pretraga

Pregled bibliografske jedinice broj: 680541

A role of Toll like receptor 7 - expressing B cells in the primary Sjögren's syndrome


Fabijanić, Iris; Markeljević Jasenka; Mravak- Stipetić Marinka; Šantak Maja; Grgić Ivana; Gorenec Lana; Vukosav, Vinka.
A role of Toll like receptor 7 - expressing B cells in the primary Sjögren's syndrome // Arthritis and Rheumatology (2013) (znanstveni, poslan)


Naslov
A role of Toll like receptor 7 - expressing B cells in the primary Sjögren's syndrome
(A role of Toll-like receptor 7 - expressing B cells in the primary Sjögren's syndrome)

Autori
Fabijanić, Iris ; Markeljević Jasenka ; Mravak- Stipetić Marinka ; Šantak Maja ; Grgić Ivana ; Gorenec Lana ; Vukosav, Vinka.

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Arthritis and Rheumatology (2013)

Status rada
Poslan

Ključne riječi
Toll like receptor 7; B cells; primary Sjögren's syndrome

Sažetak
Objective: To investigate the expression of Toll- like receptor 7 (TLR7) – signaling pathway and to determine whether TLR7 contributes to the pathogenesis of the primary Sjögren's syndrome (pSS). Methods: A quantitative polymerase chain reaction was used to analyze the expression of TLR7 mRNA from the PBMCs of 28 patients with pSS and 22 patients with chronic sialadenitis as controls. Expression of TLR7 on peripheral blood B cells was assessed by flow cytometry. Results: A significantly increased mean fluorescence intensity (MFI) of TLR7 on B cells in patients with pSS compared to control subjects (p<0.001) was found. We also demonstrated that TLR7 mRNA was significantly higher in the PBMCs of individuals with pSS than in those from the controls (p<0.001). Moreover, we investigated the relationship between laboratory parameters and the expression of TLR7 at both mRNA and protein levels. Furthermore, our findings showed that the expression of TLR7 mRNA significantly positively correlated with the presence of anti-SSA (p<0.001) and anti-SSB antibodies (p<0.001). In addition, similar results were obtained between the MFI and the presence of anti-SSA (p<0.001) and anti-SSB antibodies (p<0.001). Conclusion: Our study has provided the first evidence that innate immunity plays a role in the pathogenesis of pSS through TLR7 on B cells. In this setting, targeting TLR7 may represent a novel therapeutic approach for pSS in the near future.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081874-2416 - Sjögrenov sindrom - neurohumoralna regulacija autoimunosti i aterogeneza (Jasenka Markeljević, )

Ustanove
Medicinski fakultet, Zagreb