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Pregled bibliografske jedinice broj: 678401

First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm


Bošnjak, Ivana; Bielen, Ana; Babić, Sanja; Šver, Lidija; Topić Popović, Natalija; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelindra; Sauerborn Klobučar, Roberta
First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm // Arhiv za higijenu rada i toksikologiju, 65 (2014), 1; 67-75 doi:10.2478/10004-1254-65-2014-2421 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 678401 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm

Autori
Bošnjak, Ivana ; Bielen, Ana ; Babić, Sanja ; Šver, Lidija ; Topić Popović, Natalija ; Strunjak-Perović, Ivančica ; Čož-Rakovac, Rozelindra ; Sauerborn Klobučar, Roberta

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 65 (2014), 1; 67-75

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ABC transporter proteins ; cellular detoxification mechanism ; modulator ; MXR ; phenotype ; soil organism

Sažetak
Multixenobiotic resistance (MXR) mechanism is present in many organisms as an important cellular detoxification mechanism and is mediated by the activity of ATP binding cassette (ABC) transporters that bind and actively efflux different toxic substrates out of the cell. Here we present data on molecular identification of ABC efflux transporter - P-glycoprotein (P-gp/Abcb1), as a representative of MXR phenotype, in soil organism - earthworm *Eisenia fetida*. Partial cDNA sequence of *Abcb1 * was identified and its overall identity with ABCB1 homologues of other organisms ranged from 58.5 to 72.5 %. Furthermore we looked at the impact of five modulators (verapamil, cyclosporin A, MK571, probenecid, orthovanadate) to the earthworm’s MXR mechanism activity. To confirm MXR reversion by these agents, we measured in *E. fetida* the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. The results showed that MK571, OV and VER modulators significantly inhibit MXR transport activity in experiment performed on filter paper contact test. Our data for the first time evidence the presence of P-gp/Abcb1 related gene in earthworm "E. fetida". In addition, we have demonstrated the importance of MXR as the specific detoxification mechanism that helps earthworms to survive in polluted environments.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija



POVEZANOST RADA


Projekti:
058-0582261-2246 - Utjecaj mutagena i antimutagena na molekularne procese u stanici (Hrašćan, Reno, MZOS ) ( POIROT)
098-1782739-2749 - Substanična biokemijska i filogenetska raznolikost tkiva riba, rakova i školjaka (Čož-Rakovac, Rozelinda, MZOS ) ( POIROT)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi Hrčak www.degruyter.com

Citiraj ovu publikaciju:

Bošnjak, Ivana; Bielen, Ana; Babić, Sanja; Šver, Lidija; Topić Popović, Natalija; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelindra; Sauerborn Klobučar, Roberta
First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm // Arhiv za higijenu rada i toksikologiju, 65 (2014), 1; 67-75 doi:10.2478/10004-1254-65-2014-2421 (međunarodna recenzija, članak, znanstveni)
Bošnjak, I., Bielen, A., Babić, S., Šver, L., Topić Popović, N., Strunjak-Perović, I., Čož-Rakovac, R. & Sauerborn Klobučar, R. (2014) First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm. Arhiv za higijenu rada i toksikologiju, 65 (1), 67-75 doi:10.2478/10004-1254-65-2014-2421.
@article{article, year = {2014}, pages = {67-75}, DOI = {10.2478/10004-1254-65-2014-2421}, keywords = {ABC transporter proteins, cellular detoxification mechanism, modulator, MXR, phenotype, soil organism}, journal = {Arhiv za higijenu rada i toksikologiju}, doi = {10.2478/10004-1254-65-2014-2421}, volume = {65}, number = {1}, issn = {0004-1254}, title = {First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm}, keyword = {ABC transporter proteins, cellular detoxification mechanism, modulator, MXR, phenotype, soil organism} }
@article{article, year = {2014}, pages = {67-75}, DOI = {10.2478/10004-1254-65-2014-2421}, keywords = {ABC transporter proteins, cellular detoxification mechanism, modulator, MXR, phenotype, soil organism}, journal = {Arhiv za higijenu rada i toksikologiju}, doi = {10.2478/10004-1254-65-2014-2421}, volume = {65}, number = {1}, issn = {0004-1254}, title = {First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm}, keyword = {ABC transporter proteins, cellular detoxification mechanism, modulator, MXR, phenotype, soil organism} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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