engleski

Histological Groups of Human Postpubertal Testicular Germ Cell Tumours Harbour Different Genetic Alterations

Purpose: Testicular germ cell tumors are the most common malignacies in young males. Molecular biology studies of these tumors are often contradictory. Two histological groups, seminoma and nonseminoma, differ morphologically and in malignant behaviour. Although a common cytogenetic feature is seen, amplification of 12p chromosomal region, development mechanisms of relatively less aggressive seminoma and more aggressive nonseminoma are unknown. Methods: Occurence of structural genetic alterations was analysed in 18 seminoma and 22 nonseminoma for genes involved in a malignant tumour phenotype: CDH1, APC (cellular differentiation and adhesion) ; NME1 (cellular motility) ; TP53, CDKN2A, RB1 (cell cycle and genome integrity control) ; RAD51, MSH2, MLH1, BRCA1 (DNA damage repair) ; BAX (apoptosis) ; ABCG2 (drug resistance). Genetic alterations, loss of heterozygosity (LOH) and microsatellite instability (MSI), were analysed using restriction fragments length or microsatellite repeats length polymorphisms. Results: A difference betwen structural genetic alterations in seminoma and nonseminoma has been observed. Conclusion: Occurence of genetic alterations indicate an independent development of seminoma and nonseminoma, and correlates with clinical behaviour of those tumours.

loss of heterozygosity; seminomas; nonseminomas; TGCT

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