engleski

EXPRESSION OF Ki-67 IN CANINE CIRCUMANAL GLAND TUMORS AND NORMAL CIRCUMANAL GLAND

Introduction: Circumanal gland (CG) tumours comprise a group of common canine skin tumours that arise from the modified sebaceous glands located in the dermis of cutaneous zone of anus. The aim of this study was to detect the proliferating capacity of these tumours using Ki-67 immunohistochemical (IHC) marker. Materials and Methods: A total of 64 samples (10 normal circumanal glands, 15 CG adenomas, 11 CG epitheliomas, 21 well differentiated CG carcinomas and 7 poorly differentiated carcinomas) were analysed using Ki-67 IHC marker (MIB-1, Dako). Results: Average values of Ki-67 expressions within cells were as follows: normal CG – 5.7% ; CG adenomas – 4.9% ; CG epitheliomas – 14.4% ; well differentiated CG carcinomas – 16.4% ; poorly differentiated CG carcinomas – 20.8%. Significant differences between these values were spotted between normal CG and either well differentiated or poorly differentiated carcinomas (p<0.01) ; between CG adenomas and CG epitheliomas (p<0.05) ; between CG adenomas and either well differentiated or poorly differentiated carcinomas (p<0.01). Conclusions: The results show overall quite low Ki-67 proliferation index (PI) of these tumours. The PI values of CG epitheliomas, well differentiated CG carcinomas and poorly differentiated CG carcinomas are comparable. However, there are significant differences between malignant variants of these tumours and normal CG or CG adenoma.

circumanal glands tumors; dog; Ki-67; immunohistochemistry

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