engleski

Application of the horizontal rotating tubular bioreactor for the biofuels production: Optimisation of the enzymatic lignocellulose material treatment and bioethanol production

The horizontal rotating tubular bioreactor (HRTB) was constructed to provide maximum efficiency and minimal energy consumption in the biofuels production processes. A model of the sugar beet pulp enzymatic treatment was established to predict optimal bioprocess performance in HRTB. Furthermore, Design of Experiment (DOE) was also apply to improve the optimisation and selection of the multi-enzyme system. Different DOEs (General, Two level fractorial, Box Behnken, and D-optimal designs) were preformed in order to optimise multi-enzyme system. Obtained results were used in the simulations of bioprocess performance in the HRTB by Response Surface Methodology (RSM). From obtained results it is evident that changes in the initial lignocellulose material concentration and bioreactor rotation speed significantly influence bioprocess efficiency. The highest ethanol yield was observed by 12rpm and initial lignocellulose material concentration of 133 g/L. On the bases of DOE application and simulation of HRTB performance three assumptions made for HRTB application was confirmed: first, the high conversion yields, second, high degree of initial lignocellulose material hydrolysation, and third, reduction in enzyme amounts and costs.

bioethanol production; enzymatic treatment; Design of Experiment; Response Surface Methodology; horizontal rotating tubular bioreactor

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