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Pregled bibliografske jedinice broj: 676527

Triparental origin of triploid onion, Allium × cornutum (Clementi ex Visiani, 1842), as evidenced by molecular, phylogenetic and cytogenetic analyses


Fredotović, Željana; Šamanić, Ivica; Schneeweiss-Weiss, Hanna; Kamenjarin, Juraj; Jang, Tae-Soo; Puizina, Jasna
Triparental origin of triploid onion, Allium × cornutum (Clementi ex Visiani, 1842), as evidenced by molecular, phylogenetic and cytogenetic analyses // Bmc plant biology, 14 (2014), 24-1 doi:10.1186/1471-2229-14-24 (međunarodna recenzija, članak, znanstveni)


Naslov
Triparental origin of triploid onion, Allium × cornutum (Clementi ex Visiani, 1842), as evidenced by molecular, phylogenetic and cytogenetic analyses

Autori
Fredotović, Željana ; Šamanić, Ivica ; Schneeweiss-Weiss, Hanna ; Kamenjarin, Juraj ; Jang, Tae-Soo ; Puizina, Jasna

Izvornik
Bmc plant biology (1471-2229) 14 (2014); 24-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Allium × cornutum; Allium cepa; Allium roylei; Allium pskemense; triparental hybrid; triploid; fluorescence in situ hybridisation (FISH); ITS1-5.8S-ITS2; 5S rDNA non-transcribed spacer (NTS); genomic in situ hybridisation (GISH)

Sažetak
Reconstruction of the parental origins of cultivated plants from wild relatives, especially after long periods of domestication, is not a trivial task. However, recent advances in molecular phylogenetics, among other approaches, have proved to be very informative in analyses of the origin and evolution of polyploid genomes. An established minor garden crop, triploid onion Allium × cornutum (Clementi ex Visiani, 1842) (2n = 3x = 24), is widespread in southeastern Asia and Europe. Our previous cytogenetic analyses confirmed its highly heterozygous karyotype and indicated its possible complex triparental genome origin. Allium cepa L. and Allium roylei Stearn were suggested as two putative parental species of A. × cornutum, whereas the third parental species remained hitherto unknown. Results: Here we report the phylogenetic analyses of the internal transcribed spacers ITS1-5.8S-ITS2 of 35S rDNA and the non-transcribed spacer (NTS) region of 5S rDNA of A. × cornutum and its relatives of the section Cepa. Both ITS and NTS sequence data revealed intra-individual variation in triploid onion, and these data clustered into the three main clades, each with high sequence homology to one of three other species of section Cepa: A. cepa, A. roylei, and unexpectedly, the wild Asian species Allium pskemense B. Fedtsh. Allium pskemense is therefore inferred to be the third, so far unknown, putative parental species of triploid onion Allium × cornutum. The 35S and 5S rRNA genes were found to be localised on somatic chromosomes of A. × cornutum and its putative parental species by double fluorescent in situ hybridisation (FISH). The localisation of 35S and 5S rDNA in A. × cornutum chromosomes corresponded to their respective positions in the three putative parental species, A. cepa, A. pskemense, and A. roylei. GISH (genomic in situ hybridisation) using DNA of the three putative parental diploids corroborated the results of the phylogenetic study. Conclusions: The combined molecular, phylogenetic and cytogenetic data obtained in this study provided evidence for a unique triparental origin of triploid onion A. × cornutum with three putative parental species, A. cepa, A. pskemense, and A. roylei.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo



POVEZANOST RADA


Projekt / tema
177-0680722-3581 - Značaj sukcesije za očuvanje bioraznolikosti Mediteranske vegetacijske regije (Juraj Kamenjarin, )
177-1191196-0829 - Mehanizmi očuvanja stabilnosti genoma u viših biljaka (Jasna Puizina, )

Ustanove
Prirodoslovno-matematički fakultet, Split

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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