engleski

Molecular study of dominant soil bacteria: Streptomycetes in nature and application to biotechnology

Streptomyces coelicolor is the soil-inhabiting bacterium with a complex life style and a good model system to study streptomycetes, the best known antibiotic producing microorganisms. The genetic adaptability to a wide range of terrestrial environments is evident in the genome of S. coelicolor. The large linear chromosome of this bacterium encodes 7825 ORFs with numerous genes predicted to encode regulatory proteins. This lecture will address two aspects of Streptomyces biology. Summary of our current research on DNA metabolism that involves answering the question what is the driving force that has led to the duplication and retention of paralogous ssb genes will be presented. In addition, proposed cellular functions of SsbA and SsbB during Streptomyces growth and reproductive phase will be described. Secondly, this lecture will focus on genomics and biotechnology in order to show that streptomycetes still represent an excellent source for genome mining and new biotechnological approaches. An interesting subclass of lipolytic enzymes will be presented. This protein family possesses a distinct GDS(L) sequence motif and has multifunctional properties with potential for use in the hydrolysis and synthesis of important ester compounds of biotechnological interest. The sequencing of the genomes of several streptomycete species revealed their great potential for synthesis of a broad spectrum of lipases. Beside that our group has done intensive bioinformatic screening of GDS(L) lipases from Streptomyces and related microorganisms (Actinobacteria). Moreover, we are searching for a new Actinobacteria from wide diversity of ecological niches.

Streptomyces; ssb genes; GDS(L)-lipolytic enzymes

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