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Pregled bibliografske jedinice broj: 675638

Characterisation of the T-Cell response in the brain of CMV-infected newborn mice


Huzthy, Peter; Arapović, Jurica; Arapović, Maja; Golemac, Mijo; Pugel, Ester; Torti, Nicole; Oxenius Annette; Krmpotić, Astrid; Jonjić, Stipan
Characterisation of the T-Cell response in the brain of CMV-infected newborn mice // Book of Abstracts, Croatian Immunological Society / Polić, Bojan (ur.).
Zagreb, 2012. str. 27-27 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Characterisation of the T-Cell response in the brain of CMV-infected newborn mice

Autori
Huzthy, Peter ; Arapović, Jurica ; Arapović, Maja ; Golemac, Mijo ; Pugel, Ester ; Torti, Nicole ; Oxenius Annette ; Krmpotić, Astrid ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, Croatian Immunological Society / Polić, Bojan - Zagreb, 2012, 27-27

Skup
Annual meeting, Croatian Immunological Society, Marija Bistrica, 2012

Mjesto i datum
Marija Bistrica, Hrvatska, 05. - 06.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
brain; CMV -infection; T-cell response

Sažetak
Congenital human cytomegalovirus (HCMV) infections are the most common cause of mental retardation and hearing loss in adolescents. As HCMV does not cross the species barrier, we employed a mouse model to study brain inflammatory responses to perinatal mouse cytomegalovirus (MCMV) infection. Infection of newborn mice led to systemic virus dissemination, with virus persistence in salivary glands and lungs. In the brain of MCMV infected mice, virus titers peaked at day 14 post infection (p.i.), followed by a wave of inflammatory cells, of which CD8 T cells were the most numerous and peaked on day 21 p.i. Histological analysis revealed virus-induced cellular changes in the brain, including perivascular- and tissue-infiltrating leukocytes. In addition, we observed a virus-induced retardation in cerebellum growth, a delay in granular neuron migration and disturbances in radial glia formation. Immunohistological analysis confirmed that day 21 p.i. was the time point with the largest number of tissue-infiltrating T-cells. Phenotypic analysis showed that brain-isolated MCMV-specific CD8 T cells were highly activated, as compared to those in peripheral organs. Interestingly, during the course of MCMV infection, and also after virus clearance, the percentage of CD103 cells increased, indicating the presence of a brain-resident memory T-cell population. Furthermore, by adoptive transfer of CD8 T cells from MHC class I-restricted TCR transgenic mice with specificity for the MCMV-derived epitope M38, we demonstrated that upon viral infection, CD8 T cells migrated from the periphery to the brain of newborn mice, where they continued to expand. Importantly, adoptively transferred transgenic cells did not suppress the endogenous CD8 T cell response

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Pernjak-Pugel, Ester, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka


Citiraj ovu publikaciju

Huzthy, Peter; Arapović, Jurica; Arapović, Maja; Golemac, Mijo; Pugel, Ester; Torti, Nicole; Oxenius Annette; Krmpotić, Astrid; Jonjić, Stipan
Characterisation of the T-Cell response in the brain of CMV-infected newborn mice // Book of Abstracts, Croatian Immunological Society / Polić, Bojan (ur.).
Zagreb, 2012. str. 27-27 (poster, međunarodna recenzija, sažetak, znanstveni)
Huzthy, P., Arapović, J., Arapović, M., Golemac, M., Pugel, E., Torti, N., Oxenius Annette, Krmpotić, A. & Jonjić, S. (2012) Characterisation of the T-Cell response in the brain of CMV-infected newborn mice. U: Polić, B. (ur.)Book of Abstracts, Croatian Immunological Society.
@article{article, editor = {Poli\'{c}, B.}, year = {2012}, pages = {27-27}, keywords = {brain, CMV -infection, T-cell response}, title = {Characterisation of the T-Cell response in the brain of CMV-infected newborn mice}, keyword = {brain, CMV -infection, T-cell response}, publisherplace = {Marija Bistrica, Hrvatska} }
@article{article, editor = {Poli\'{c}, B.}, year = {2012}, pages = {27-27}, keywords = {brain, CMV -infection, T-cell response}, title = {Characterisation of the T-Cell response in the brain of CMV-infected newborn mice}, keyword = {brain, CMV -infection, T-cell response}, publisherplace = {Marija Bistrica, Hrvatska} }




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