The origin of increased serum enzyme activities in pigs exposed to physical exercise (CROSBI ID 606574)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
SALAJPAL, Kresimir ; ĐIKIC, Marija ; KAROLYI, Danijel
engleski
The origin of increased serum enzyme activities in pigs exposed to physical exercise
Creatine kinase (CK) and lactate dehydrogenase (LDH) are two main enzymes in the serum of pigs which can be markedly increased as a result skeletal or heart muscle damage. In this study the effect of physical exercise on serum enzymes activity and their origin were investigated. The study was conducted on a total of 25 commercial fattened pigs of 6 mounts of age and 105-110 kg of live weight. Pigs were exposure to physical exercise during forced transport procedure (loading, transport and unloading) which lasted about 2 hours. Blood samples were collected immediately prior the loading and after unloading and total serum enzymes activity of creatine kinase (CK), lactate dehydrogenase (LDH) and their isoenzymes (CKMB, LDH1-5) were analyzed. Physical exercise caused significantly increase in CK and LDH activity (P<0.05) in serum of pig. The 30% increase in total CK and 20% increase in CKMB activity were observed. In addition, 10% increase in total LDH activity comes as a result of increased activity of LDH5 fraction (20%) originates from skeletal muscle tissue, while changes in activity of other LDH isoenzymes (LDH1-4) were much smaller or nonexistent (<5%). On the basis of these results we could conclude that physical exercise caused markedly increase in serum CK and LDH activity as a result of their release from muscle cells.
Pig; physical excerise; LDH; CK; isoenzymes
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Podaci o prilogu
24-24.
2013.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstract X X I A n i m a l S c i e n c e D a y s E U R O P E A N R E S E A R C H P R I O R I T I E S I N ANIMAL SCIENCE
Podaci o skupu
XXI Animal Science Days
poster
18.09.2013-20.09.2013
Padova, Italija