engleski

Hydrothermal and biocatalytic conversion of lignocellulosic renweable resources

Goal of this study was to optimize pre-treatment conditions for beech wood to remove lignin and/or hemicellulose from cellulosic fibers, thus making them more accessible for cellulolytic enzymes. Focusing on "Organosolv"- and "Liquid Hot Water" extraction, the effect of temperature, extraction time, liquid to solid ratio and the combination of both methods have been investigated. As measurable factors for successful extraction were the glucose concentration after enzymatic hydrolysis of the gained fiber at 200 g*L-1 and concentrations of the potential inhibitors HMF and furfural. It was concluded that longer pretreatment time in combination with higher liquid to solid ratio results in higher glucose yield after enzymatic hydrolysis and higher HMF and furfural concentrations in pretreatment liquor. Furthermore, enzymatic hydrolysis under high pressures, and pressure impact on the stabilization of Cellic® CTec2 enzymes were studied. The economic potential of biorefineries could be considerably improved when hydrolysis time under high pressure will be reduced. The results obtained in these experiments show that high pressure has positive impact on the enzymatic activity and consequently higher sugar concentrations were observed after hydrolysis.

LHW; Organosolv; high pressure hydrolysis; biorefineries; beech wood

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