engleski

Airborne moulds in grain mill and dwellings with special attention to Aspergilli: a pilot study

The purpose of this study was to assess composition and concentration of aeromycota in the grain mill and dwellings in comparison to outdoor air. All samples were collected during January 2012 using Airsampler MAS 100 Eco (Merck) and Dichlorane 18% Glycerol agar (DG-18). After sampling the plates were incubated at 25 ⁰C for five days in the dark and colony forming units in cubic meter were counted (CFU/m3). Aspergillus species were isolated on Czapek Yeast Agar (CYA) and Malt Extract Agar (MEA) and incubated at 25 ⁰C in the dark for seven days in order to separate Aspergilli of interest: Aspergillus section Flavi, section Nigri and section Versicolores. The identification of Aspergillus species was made according to Pitt and Hocking, 2009 and Samson and Frisvad, 2004. Airborne moulds were counted as follows: 25630 CFU/m3 (grain mill), 163 CFU/m3 (flat), 697 CFU/m3 (basement) and 286 CFU/m3 (outdoors). Aspergillus species were prevalent species in all examined samples: 100% mill, 95% basement, 80% flat and 90 % outdoor samples. Eurotium spp. were also dominant species in the samples (90% mill, 60% flat, 40 % basement and 50% outdoor samples). Aspergilli of section Flavi were the most prevalent in the mill samples (80%) and were not present in the samples from outdoor or dwellings (flat and basement). Black Aspergilli were isolated in 40% of mill samples and in 5 % of samples taken in the dwellings (flat and basement) while these species were not isolated from the samples of outdoor air. Aspergilli of section Versicolores were isolated from 30% of flat and 45% of basement samples and were not found in the mill or the outdoor samples. The other mould genera prevalent in the samples were Penicillium spp. (100% mill, 90% flat, 85% basement, 90% outdoors) and Cladosporium spp. (60 % mill, 45 % flat, 55 % basement and 100 % outdoors) followed by Ulocladium spp. (20 % flat and 50% basement) , Wallemia spp. (30 % flat, 30 % basement and 10 % outdoors), Alternaria spp. (10 % mill, 15 % flat, 25 % basement and 20 % outdoors), Epicoccum spp. (10% mill and 5% basement), Rhizopus spp. (10 % mill), Botrytis spp. (5% basement), Chrysonillia spp. (5% basement) and Aureobasidium spp. (5% outdoors). This study will be continued during the year 2012 in order to assess seasonal variations in composition of aeromycota regarding to differences in sampling environments.

Airborne fungi; Aspergillus; sections Flavi; Nigri; Veriscolores

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