engleski

Nanosilver particles versus ionic silver – chromatographic differentiation and quantificationN

Research and development in the area of new nanomaterials containing silver nanoparticles (AgNPs) are seen as having an enormous economic potential for new drugs and medical treatments, electronics, environmental remediation, surface treatments, etc. owing to their excellent antibacterial properties s. To date, little is understood concerning the distribution, accumulation, and target organs of AgNPs in organisms. Moreover, there have been some reports indicating that AgNPs move into the brain by traversing the blood-brain barrier. The size of a particle and its differentiation from ionic metal form are key parameters to be determined when attempting to predict the fate, and potential toxicological impact, of silver NPs released into the environment. Analytical techniques based on mass spectrometry (MS) can provide both elemental and molecular information. Recently, ICP-MS has been demonstrated to be a highly valuable tool for ultrasensitive detection and characterization of metalloid-containing NPs. Size distribution analysis of NPs and differentiation of nanoparticulate and ionic form in polydispersed samples could be achieved with liquid chromatography. Several recent studies presented the separation and quantitative determination of Ag species using high-performance liquid chromatography (HPLC) coupled interfaced to a multi-element detector (ICPMS). This could be a novel bioanalytical tool that enables investigations into the behaviour and fate of a range of inorganic silver NP's in ‘realworld’ situations. We present a validation and evaluation of the proposed methods for differentiation and quantification of ionic and nano form of silver. Reproducibility and robustness of the LC-ICPMS methodology were evaluated for a range of silver ions and AgNPs in different sample matrices in order to test the suitability for routine applications.

silver; nanoparticles; stability; chromatography; separation

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