engleski

Analysis of renal ganglioside GM3 distribution in rat models of type 1 and type 2 diabetes

Ganglioside GM3 is particularly abundant in the kidney tissue and is thought to play an important role in the maintenance of the charge-selective filtration barrier of glomeruli. Considering the role of GM3 ganglioside in kidney function, the aim of this study was immunohistochemical analysis of renal GM3 distribution in rat models of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). Diabetes was induced with streptozotocin (55 mg/kg for T1DM, n=5 ; and 35 mg/kg for T2DM, n=5) injection to male Sprague-Dawley rats weighing between 140 and 160 g which were fed with normal pellet diet (T1DM) or high-fat diet (T2DM). Rats were sacrified two weeks after diabetes induction, frozen renal sections were stained with primary GM3 antibody and visualized by secondary antibody coupled with Texas-Red. Immunofluorescent microscopy detected 1.7 fold higher GM3 expression in tubules and 1.25 fold higher GM3 in glomeruli of T1DM compared to control group. T2DM rats showed unchanged GM3 in glomeruli, but significant higher GM3 expression in tubules, compared to control animals. Given the known role of GM3 ganglioside in kidney function and our finding of increased tubular GM3 content in both types of diabetes, we could hypothesize the role of GM3 in early pathogenesis of diabetic nephropathy.

rat; diabetes mellitus; streptozotocin; kidney; GM3

nije evidentirano