engleski

TLR-4 GENE POLYMORPHISM IN RENAL TRANSPLANT PATIENTS

The study included 70 patients with a transplanted kidney and 50 healthy volunteers representing a control group. Median age of the transplanted patients was 53 years (48-58 years). Transplanted patients had been receiving an adjuvant kidney therapy for an average of 4 years (2-6 years). Median time lapse from receiving kidney transplant was 3 years (ranging from 2-5years). The most common chronic kidney disease diagnosis was chronic glomerulonephritis (21 patient ; 32, 8%), followed by a polycystic kidney disease in 7 patients (10, 9%). Detection of gene polymorphism for tlr4 rs4986790 (A896G, D299G) i tlr4 rs4986791 (C1196T, T399I) was conducted using multiplex real-time PCR. Polymorphism detection was performed using LightCycler. Detection of the TLR4 rs4986790 polymorphism revealed a great predominance in the AA-wild type (104 patients, 86.7%), 44 (88%) out of which were from the control group and 60 (85.7%) from the transplanted group. When analysing rs4986791 polymorphism, the largest proportion of patients (99 ; 82.5%) displays a CC-wild type, followed by a CT-heterozygous group (19 ; 15, 8%), while only 2 patients from the tranplanted group (2.9%) showed a TT-mutation. A significant decrease in erythrocytes (Kruskal Wallis test, p=0.037), creatinine clearence (p=0.019) and hemoglobin levels (p=0.009) was observed in the AA-wild type rs4984790 group, while the AG-heterozygous group showed the highest levels of blood glucose. CC-wild type group type rs4986791 shows significantly lower erythrocyte values (p=0.027), creatinine clearance (p=0.030) and hemoglobin levels (p=0.049).

kidney transplantation; graft function; TLR4

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