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Pregled bibliografske jedinice broj: 670738

NDM-1-producing Klebsiella pneumoniae, Croatia


Mazzariol, Annarita; Bošnjak, Zrinka; Ballarini, Piero; Budimir, Ana; Bedenić, Branka; Kalenić, Smilja; Cornaglia, Giuseppe
NDM-1-producing Klebsiella pneumoniae, Croatia // Emerging infectious diseases, 18 (2012), 3; 532-534 doi:10.3201/eid1803.110389 (podatak o recenziji nije dostupan, pismo uredniku, znanstveni)


CROSBI ID: 670738 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
NDM-1-producing Klebsiella pneumoniae, Croatia

Autori
Mazzariol, Annarita ; Bošnjak, Zrinka ; Ballarini, Piero ; Budimir, Ana ; Bedenić, Branka ; Kalenić, Smilja ; Cornaglia, Giuseppe

Izvornik
Emerging infectious diseases (1080-6040) 18 (2012), 3; 532-534

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pismo uredniku, znanstveni

Ključne riječi
Klebsiella pneumoniae ; NDM-1

Sažetak
Objectives: A K. pneumoniae strain, resistant to carbapenems, was isolated in May 2009, from a blood culture of a 40-years-old man, recovered in a surgical intensive care unit of the Clinical Hospital Center in Zagreb, Croatia.The patient had been transferred from another hospital of a neighbouring country after 5 days of hospitalization due to a car accident. The clinical history mentioned antibiotic treatment not including carbapenems (gentamicine, metronidazole and ceftriaxone), and no link to India or Pakistan.We investigated the carbapenem resistance mechanism. Materials and Methods: Antimicrobial susceptibility testing was performed by both Vitek2 and microdiluition, and interpreted according to the latest EUCAST documents. The presence of a carbapenemase was investigated by means of an MBL-etest, hydrolysis of carbapenems and EDTA inhibition of hydrolysis. PCR and sequencing were carried out by standard procedures. Resistance was transferred by conjugation to E. coli J53. Results: The strain was resistant to imipenem and meropenem (MICs 16 g/ml and 64 g/ml, respectively), and to all 3G-cephalosporins (MICs>128 g/ml), and susceptible to ciprofloxacin (MIC 0.25 g/ml) It also harboured a qnrA and a CTX-M-15 genes. The presence of an MBL was suggested by the MBL Etest and confirmed spectrophotometric by EDTA-inhibited imipenem hydrolysis. PCR performed by using primers specific for blaIMP, , blaVIM, blaSPM, blaGIM, and blaSIM, did not yield any product. A product obtained with NDM primers was confirmed, after sequencing, to code for an NDM-1 enzyme. The resistance to carbapenems and 3G-cephalosporins was transferred to E. coli J53 by conjugation. Conclusions: To our knowledge this is the first report of an NDM-1-producing K. pneumoniae in Croatia and in the Balkans. The patient had no apparent link to the Indian subcontinent and the strain was isolated before the recent outbreak of NDM-1 producing Enterobacteriaceae. Although several cases reported by a recent pan-European survey showed a clear link to the Balkan region, this is the first documented report of NDM-1 from that area, thus confirming a possible multifocal spread of this enzyme and prompting for a widespread epidemiological surveillance.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
108-1080114-0015 - Mehanizmi rezistencije na antibiotike u Gram-negativnih bakterija (Bedenić, Branka, MZOS ) ( POIROT)
108-1080114-0306 - Djelovanje antibiotika na uzročnike biofilm infekcija

Ustanove:
Sveučilište u Zagrebu

Profili:

Avatar Url Branka Bedenić (autor)

Avatar Url Smilja Kalenić (autor)

Avatar Url Ana Budimir (autor)

Avatar Url Zrinka Bošnjak (autor)

Citiraj ovu publikaciju

Mazzariol, Annarita; Bošnjak, Zrinka; Ballarini, Piero; Budimir, Ana; Bedenić, Branka; Kalenić, Smilja; Cornaglia, Giuseppe
NDM-1-producing Klebsiella pneumoniae, Croatia // Emerging infectious diseases, 18 (2012), 3; 532-534 doi:10.3201/eid1803.110389 (podatak o recenziji nije dostupan, pismo uredniku, znanstveni)
Mazzariol, A., Bošnjak, Z., Ballarini, P., Budimir, A., Bedenić, B., Kalenić, S. & Cornaglia, G. (2012) NDM-1-producing Klebsiella pneumoniae, Croatia. Emerging infectious diseases, 18 (3), 532-534 doi:10.3201/eid1803.110389.
@article{article, year = {2012}, pages = {532-534}, DOI = {10.3201/eid1803.110389}, keywords = {Klebsiella pneumoniae, NDM-1}, journal = {Emerging infectious diseases}, doi = {10.3201/eid1803.110389}, volume = {18}, number = {3}, issn = {1080-6040}, title = {NDM-1-producing Klebsiella pneumoniae, Croatia}, keyword = {Klebsiella pneumoniae, NDM-1} }
@article{article, year = {2012}, pages = {532-534}, DOI = {10.3201/eid1803.110389}, keywords = {Klebsiella pneumoniae, NDM-1}, journal = {Emerging infectious diseases}, doi = {10.3201/eid1803.110389}, volume = {18}, number = {3}, issn = {1080-6040}, title = {NDM-1-producing Klebsiella pneumoniae, Croatia}, keyword = {Klebsiella pneumoniae, NDM-1} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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