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Pregled bibliografske jedinice broj: 669980

The stability of ethanol in unstoppered tubes


Saračević, Andrea; Šimundić, Ana-Maria; Dukić, Lora
The stability of ethanol in unstoppered tubes // Clinical biochemistry, 47 (2014), 1/2; 92-95 doi:10.1016/j.clinbiochem.2013.11.006 (međunarodna recenzija, članak, znanstveni)


Naslov
The stability of ethanol in unstoppered tubes

Autori
Saračević, Andrea ; Šimundić, Ana-Maria ; Dukić, Lora

Izvornik
Clinical biochemistry (0009-9120) 47 (2014), 1/2; 92-95

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Ethanol ; storage conditions ; sample stability ; preanalytical phase

Sažetak
The exact time frame within which ethanol can be reliably measured in unstoppered tubes is not known. The aim of this study was to investigate the stability of alcohol concentration in unstoppered tubes. 44 samples with alcohol concentration > 2.7 mmol/L were included in the study. Measurements were done on Vitros 250 analyzer with original Vitros reagents. After the initial alcohol measurement, each sample was aliquoted into two separate clean tubes (1 mL). One of the aliquoted tubes was stoppered immediately after aliquoting and remained stoppered during the experiment ; while the other two tubes (original sample tube and the second aliquoted tube) remained open. During the experiment all three tubes were kept at room temperature. Alcohol concentration was measured at 30 minutes, 1, 2 and 3 hours after the initial measurement in all 3 tubes. The differences between the time intervals for each test tubes were examined using repeated measures Anova or Friedman test. The deviation from the initial concentration was calculated for all three test tubes for each time interval. The calculated deviations were compared with desirable imprecision specifications (DI) according to the RiliBÄK (DI < 9%). Results We found a statistically significant difference between the initial concentration and the concentration in unstoppered tubes for all the investigated time intervals ; however, the DI was exceeded only in the original tube and in the tube B, 3 hours after the initial measurement (− 9.2% and − 12.6%, respectively). Alcohol concentration can be accurately measured in the unstoppered samples within two hours upon decapping the tube, when stored at room temperature. Longer storage time (> 2 hours) in the unstoppered samples introduces significant bias in alcohol concentration.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
134-1340227-0200 - Upala i udio farmakogenetike u razvoju i ishodu akutnih i kroničnih bolesti (Ana-Maria Šimundić, )

Ustanove
KBC "Sestre Milosrdnice"

Citiraj ovu publikaciju

Saračević, Andrea; Šimundić, Ana-Maria; Dukić, Lora
The stability of ethanol in unstoppered tubes // Clinical biochemistry, 47 (2014), 1/2; 92-95 doi:10.1016/j.clinbiochem.2013.11.006 (međunarodna recenzija, članak, znanstveni)
Saračević, A., Šimundić, A. & Dukić, L. (2014) The stability of ethanol in unstoppered tubes. Clinical biochemistry, 47 (1/2), 92-95 doi:10.1016/j.clinbiochem.2013.11.006.
@article{article, year = {2014}, pages = {92-95}, DOI = {10.1016/j.clinbiochem.2013.11.006}, keywords = {ethanol, storage conditions, sample stability, preanalytical phase}, journal = {Clinical biochemistry}, doi = {10.1016/j.clinbiochem.2013.11.006}, volume = {47}, number = {1/2}, issn = {0009-9120}, title = {The stability of ethanol in unstoppered tubes}, keyword = {ethanol, storage conditions, sample stability, preanalytical phase} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • BIOBASE
  • Reference Update


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