Napredna pretraga

Pregled bibliografske jedinice broj: 669905

In vivo identification of periodontal progenitor cells


Roguljić, Hrvoje; Matthews, Brya; Yang, W.; Cvija, Hrvoje; Mina, Mina; Kalajzić, Ivo
In vivo identification of periodontal progenitor cells // Journal of dental research, 92 (2013), 8; 709-715 doi:10.1177/0022034513493434 (međunarodna recenzija, članak, znanstveni)


Naslov
In vivo identification of periodontal progenitor cells

Autori
Roguljić, Hrvoje ; Matthews, Brya ; Yang, W. ; Cvija, Hrvoje ; Mina, Mina ; Kalajzić, Ivo

Izvornik
Journal of dental research (0022-0345) 92 (2013), 8; 709-715

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cre recombinase; GFP; cementoblasts; periodontal ligament; scleraxis; α-smooth muscle actin

Sažetak
The periodontal ligament contains progenitor cells ; however, their identity and differentiation potential in vivo remain poorly characterized. Previous results have suggested that periodontal tissue progenitors reside in perivascular areas. Therefore, we utilized a lineage-tracing approach to identify and track periodontal progenitor cells from the perivascular region in vivo. We used an alpha-smooth muscle actin (αSMA) promoter-driven and tamoxifen-inducible Cre system (αSMACreERT2) that, in combination with a reporter mouse line (Ai9), permanently labels a cell population, termed 'SMA9'. To trace the differentiation of SMA9-labeled cells into osteoblasts/cementoblasts, we utilized a Col2.3GFP transgene, while expression of Scleraxis-GFP was used to follow differentiation into periodontal ligament fibroblasts during normal tissue formation and remodeling following injury. In uninjured three-week-old SMA9 mice, tamoxifen labeled a small population of cells in the periodontal ligament that expanded over time, particularly in the apical region of the root. By 17 days and 7 weeks after labeling, some SMA9-labeled cells expressed markers indicating differentiation into mature lineages, including cementocytes. Following injury, SMA9 cells expanded, and differentiated into cementoblasts, osteoblasts, and periodontal ligament fibroblasts. SMA9-labeled cells represent a source of progenitors that can give rise to mature osteoblasts, cementoblasts, and fibroblasts within the periodontium.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1080229-0142 - Molekularni mehanizmi učinaka imunosnih poremećaja na kost (Danka Grčević, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • MEDLINE


Citati