Napredna pretraga

Pregled bibliografske jedinice broj: 669742

Analysis of αSMA-Labeled Progenitor Cell Commitment Identifies Notch Signaling as an Important Pathway in Fracture Healing


Matthews, Brya; Grčević, Danka; Wang, L.; Hagiwara, Y.; Roguljic, Hrvoje; Joshi, P.; Shin, D.G.; Adams, Douglas; Kalajzić, Ivo
Analysis of αSMA-Labeled Progenitor Cell Commitment Identifies Notch Signaling as an Important Pathway in Fracture Healing // Journal of bone and mineral research, 29 (2014), 5; 1283-1294 doi:10.1002/jbmr.2140 (međunarodna recenzija, članak, znanstveni)


Naslov
Analysis of αSMA-Labeled Progenitor Cell Commitment Identifies Notch Signaling as an Important Pathway in Fracture Healing

Autori
Matthews, Brya ; Grčević, Danka ; Wang, L. ; Hagiwara, Y. ; Roguljic, Hrvoje ; Joshi, P. ; Shin, D.G. ; Adams, Douglas ; Kalajzić, Ivo

Izvornik
Journal of bone and mineral research (0884-0431) 29 (2014), 5; 1283-1294

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bone healing; progenitor cells; Notch

Sažetak
Fracture healing is a regenerative process that involves coordinated responses of many cell types, but characterization of the roles of specific cell populations in this process has been limited. We have identified alpha smooth muscle actin (αSMA) as a marker of a population of mesenchymal progenitor cells in the periosteum that contributes to osteochondral elements during fracture healing. Using a lineage tracing approach, we labeled αSMA-expressing cells, and characterized changes in the periosteal population during the early stages of fracture healing by histology, flow cytometry and gene expression profiling. In response to fracture, the αSMA-labeled population expanded, and began to differentiate towards the osteogenic and chondrogenic lineages. The frequency of mesenchymal progenitor cell markers such as Sca1 and PDGFRα increased after fracture. By six days after fracture, genes involved in matrix production and remodeling were elevated. In contrast, genes associated with muscle contraction and Notch signaling were downregulated after fracture. We confirmed that activating Notch signaling in αSMA-labeled cells inhibited differentiation into osteogenic and adipogenic lineages in vitro and ectopic bone formation in vivo. By characterizing changes in a selected αSMA-labeled progenitor cell population during fracture callus formation, we have shown that modulation of Notch signaling may determine osteogenic potential of αSMA-expressing progenitor cells during bone healing.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1080229-0142 - Molekularni mehanizmi učinaka imunosnih poremećaja na kost (Danka Grčević, )

Ustanove
Medicinski fakultet, Zagreb,
Medicinski fakultet, Osijek

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati