engleski

Proteomics profiling of keratocystic odontogenic tumours reveals AIDA as novel biomarker candidate

Background. Keratocystic odontogenic tumor (KCOT) is a benign, yet aggressive odontogenic tumor. Herein, proteome analysis of KCOT lesions in comparison with control patient-matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented. Methods. For the proteomics profiling, two complementary proteomics techniques MALDI-MS/MS and LC-ESI-MS/MS were employed. Potential candidate biomarkers were validated by immunohistochemistry. Results. More than 43 proteins were found to be differentially expressed or up-regulated in KCOT lesions in comparison with patient-matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re-organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signaling pathways. Conclusions. Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization- antagonist (AIDA), known as a protein that blocks activation of JNK signalling pathway , as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalet intraoseal lesions inflammatory odontogenic cysts.

AIDA; biomarkers; immunohistochemistry; keratocystic odontogenic tumor; mass

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