engleski

2D versus 3D cell culture platforms for screening antitumoral activity of novel chemical entities

Conventional two-dimensional (2D) cell cultures are not able to completely describe malignant phenotype that is related to tumorigenicity in vivo. In 2D culture conditions cells need to adapt to a flat and rigid surface that can result in altered cell metabolism and functionality. Thus, cellular functions and physiological responses present in organs are often lost in 2D cell cultures which are usual standard for testing new chemical entities (NCE). Since 3D structure and environment are crucial for cellular behaviour and tumorogenicity, recently more researchers are adopting 3D cell culture systems to obtain biological insights that are lost in 2D cell culture testing platforms as well as to overcome difference in cell morphology, proliferation and differentiation. In the case of breast cancer, 3D cell cultures represent a model for understanding cell proliferation regulation. Furthermore breast cancer cells grown in 3D can be more resistant to the same doses of chemotherapeutic substances then cells grown in 2D.

3D cell cultures; anticancer actiovity; screening

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