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Pregled bibliografske jedinice broj: 665634

Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro


Mužić, Vedrana; Katušić Bojanac, Ana; Jurić-Lekić, Gordana; Himelreich, Marta; Tupek, Katarina; Šerman, Ljiljana; Marn, Nina; Sinčić, Nino; Vlahović, Maja; Bulić-Jakuš, Floriana;
Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro // Croatian medical journal, 54 (2013), 5; 489-495 doi:10.3325/cmj.2013.54.489 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 665634 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro

Autori
Mužić, Vedrana ; Katušić Bojanac, Ana ; Jurić-Lekić, Gordana ; Himelreich, Marta ; Tupek, Katarina ; Šerman, Ljiljana ; Marn, Nina ; Sinčić, Nino ; Vlahović, Maja ; Bulić-Jakuš, Floriana ;

Izvornik
Croatian medical journal (0353-9504) 54 (2013), 5; 489-495

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
limb bud ; in vitro culture ; rat ; 5-azacytidine

Sažetak
Aim was to establish an organotypic in vitro model of limb bud development to verify whether epigenetic drug and teratogen 5-azacytidine (5azaC) has an effect on limb buds independent of its effects on the placenta. Fischer strain rat fore- and hindlimb buds were microsurgically isolated from 13 days old embryos and cultivated in vitro for two weeks at the air-liquid interface in Eagle's minimum essential medium (MEM) with 50% rat serum. 30 μmol of 5azaC was added to the fresh medium. Overall growth was measured by an ocular micrometer. Routine histology, immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), and stereological quantification of PCNA expression were performed. RESULTS: At four time points, significantly lower overall growth was detected for fore- and hindlimb bud explants cultivated with 5azaC in comparison to controls. After the culture period, numerical density of the PCNA signal for both types of limb buds was lower than for controls (P<0.001). Limb buds were initially covered by immature epithelium and contained mesenchyme, myotubes, single hemangioblasts, hemangioblast aggregates, blood islands, and capillaries. Regardless of the treatment, cartilage and epidermis differentiated, but cells and structures typical for vasculogenesis disappeared. CONCLUSION: Our findings, obtained outside of the maternal organism, stress the importance of compromised cell proliferation for 5azaC impact on limb buds. This investigation points to the necessity to establish alternatives to in vivo research on animals using teratogenic agents.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
108-1080399-0335 - Eksperimentalni embrionalni tumori i razvoj zametaka sisavaca in vitro i in vivo (Jakuš, Florijana, MZOS ) ( POIROT)
108-1080399-0383 - Muški i ženski spolni sustav: razvoj, normalna histofiziologija i neplodnost (Ježek, Davor, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Citiraj ovu publikaciju

Mužić, Vedrana; Katušić Bojanac, Ana; Jurić-Lekić, Gordana; Himelreich, Marta; Tupek, Katarina; Šerman, Ljiljana; Marn, Nina; Sinčić, Nino; Vlahović, Maja; Bulić-Jakuš, Floriana;
Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro // Croatian medical journal, 54 (2013), 5; 489-495 doi:10.3325/cmj.2013.54.489 (međunarodna recenzija, članak, znanstveni)
Mužić, V., Katušić Bojanac, A., Jurić-Lekić, G., Himelreich, M., Tupek, K., Šerman, L., Marn, N., Sinčić, N., Vlahović, M., Bulić-Jakuš, F. & (2013) Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro. Croatian medical journal, 54 (5), 489-495 doi:10.3325/cmj.2013.54.489.
@article{article, year = {2013}, pages = {489-495}, DOI = {10.3325/cmj.2013.54.489}, keywords = {limb bud, in vitro culture, rat, 5-azacytidine}, journal = {Croatian medical journal}, doi = {10.3325/cmj.2013.54.489}, volume = {54}, number = {5}, issn = {0353-9504}, title = {Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro}, keyword = {limb bud, in vitro culture, rat, 5-azacytidine} }
@article{article, year = {2013}, pages = {489-495}, DOI = {10.3325/cmj.2013.54.489}, keywords = {limb bud, in vitro culture, rat, 5-azacytidine}, journal = {Croatian medical journal}, doi = {10.3325/cmj.2013.54.489}, volume = {54}, number = {5}, issn = {0353-9504}, title = {Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro}, keyword = {limb bud, in vitro culture, rat, 5-azacytidine} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • PubMedCentral


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