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Pregled bibliografske jedinice broj: 664817

Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms


Perković, Sanja; Bašić-Kinda, Sandra; Aurer, Igor; Ugrina, Ivo; Duletić-Načinović, Antica; Lozić, Dominik; Batinić, Drago
Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms // International journal of hematology, 98 (2013), 5; 589-596 doi:10.1007/s12185-013-1432-7 (međunarodna recenzija, članak, znanstveni)


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Naslov
Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms

Autori
Perković, Sanja ; Bašić-Kinda, Sandra ; Aurer, Igor ; Ugrina, Ivo ; Duletić-Načinović, Antica ; Lozić, Dominik ; Batinić, Drago

Izvornik
International journal of hematology (0925-5710) 98 (2013), 5; 589-596

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
flow cytometry; composite lymphoma; lymphoma non-Hodgkin; immunoglobulin light chains; hematology

Sažetak
Composite mature B-cell lymphoproliferative neoplasms are rare entities characterized by the simultaneous presence of two or more distinctive B-cell derived monoclonal malignancies. This retrospective study used multiparametric flow cytometric analysis aimed at immunophenotypic profiling of composite mature B-cell lymphoproliferative neoplasms in a cohort of 413 subsequent patients with de novo leukemic B-cell chronic lymphoproliferative disorders diagnosed in our institution during a 30-month period. Biclonality was found in 16 (3.9 %)patients. The vast majority (88 %) of the cases had one of the clones phenotypically corresponding to chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). Only when composite cases were categorized by phenotype of the non-CLL/SLL malignant population did we find a statistically significant (P = 0.001) higher frequency of biclonality among cases with hairy cell leukemia (22 %). Biclonal cases had the overall B-cell membrane j to k ratio within the normal range (median, 1.9 ; reference interval 0.5–4.0), making recognition of malignancy somewhat challenging. Our analysis strategy was therefore based on the detection of aberrant B-cell phenotypes, with subsequent confirmation of the monoclonal nature of neoplastic clones with regards to light chain restriction analysis. Discrimination of the coexisting clones in biclonal cases was possible on the basis of the expression of other antigen(s) (63 %), light scatter properties (44 %), different surface light chain restriction (69 %) and/or pattern of expression (44 %). The most informative cell surface antigens proved to be CD22, CD20, surface IgM, and CD23. In conclusion, historic j/k ratio is not a reliable approach and is a poor measurement for the detection of composite lymphomas. More creative analysis techniques should be utilized for this purpose.

Izvorni jezik
Engleski

Znanstvena područja
Matematika, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
214-1081347-0355 - Uloga PI3K/Akt i MAPK u regulaciji kemorezistencije leukemijskih stanica (Drago Batinić, )
108-1081872-1908 - Dijagnostika i liječenje limfoma (Igor Aurer, )
062-1081872-0091 - Angiogeneza u agresivnih ne-Hodgkinovih limfoma (Antica Duletić-Načinović, )

Ustanove
Prirodoslovno-matematički fakultet, Matematički odjel, Zagreb,
Medicinski fakultet, Rijeka,
Medicinski fakultet, Zagreb,
KBC Split,
Klinički bolnički centar Zagreb,
Klinički bolnički centar Rijeka

Citiraj ovu publikaciju

Perković, Sanja; Bašić-Kinda, Sandra; Aurer, Igor; Ugrina, Ivo; Duletić-Načinović, Antica; Lozić, Dominik; Batinić, Drago
Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms // International journal of hematology, 98 (2013), 5; 589-596 doi:10.1007/s12185-013-1432-7 (međunarodna recenzija, članak, znanstveni)
Perković, S., Bašić-Kinda, S., Aurer, I., Ugrina, I., Duletić-Načinović, A., Lozić, D. & Batinić, D. (2013) Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms. International journal of hematology, 98 (5), 589-596 doi:10.1007/s12185-013-1432-7.
@article{article, year = {2013}, pages = {589-596}, DOI = {10.1007/s12185-013-1432-7}, keywords = {flow cytometry, composite lymphoma, lymphoma non-Hodgkin, immunoglobulin light chains, hematology}, journal = {International journal of hematology}, doi = {10.1007/s12185-013-1432-7}, volume = {98}, number = {5}, issn = {0925-5710}, title = {Multiparameter flow cytometry is necessary for detection, characterization and diagnosis of composite mature B-cell lymphoproliferative neoplasms}, keyword = {flow cytometry, composite lymphoma, lymphoma non-Hodgkin, immunoglobulin light chains, hematology} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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