Dynasore does not inhibit tne endocytosis of transferrin but impairs its endosomal trafficking in HeLa cell line (CROSBI ID 604584)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Blagojević Zagorac, Gordana ; Mahmutefendić, Hana ; Ilić Tomaš, Maja ; Karleuša, Ljerka ; Lučin, Pero
engleski
Dynasore does not inhibit tne endocytosis of transferrin but impairs its endosomal trafficking in HeLa cell line
Transferrin (Tf) is an iron-binding glycoprotein (molecular weight 80 kDa) that takes role in iron- uptake in cells and therefore regulates the free iron level in our body. Transferrin, loaded with iron, binds to the surface expressed transferrin- receptor (TfR) and enters the cell through receptor mediated, clathrin-dependent endocytosis that requires the unimpaired function of protein dynamin. Inside the cell Tf/TfR complex is directed to early endosomes where iron is released. On the other hand, apoTf/TfR is subsequently targeted to the plasma membrane either directly or indirectly via juxtanuclear recycling compartment. Although the main endocytic pathway of Tf/TfR has been extensively investigated, a lot of data are rather controversial. Considering that, the aim of this study was to better elucidate the less known links of its intracellular trafficking in HeLa cell line. To achieve that goal we used a palette of inhibitors that remodel cellular endosomal network and have followed internalized molecules by flow cytometry and confocal microscopy. Aluminum fluoride, but not dynasore, retards the endocytosis of Tf/TfR. However, both inhibitors (together with PLD and PLA inhibitors) affect the structure and function (e.g. recycling) of endosomal comaprtments. These results implicate that dynamin is not essential for endocytosis of Tf/TfR but is important for its recycling. Furthermore, small Arf GTPases are involved both in endocytosis and recycling of Tf/TfR.
transferrin; Dynasore; endocytosis
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
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Podaci o prilogu
2013.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
3rd congress of Croatian Physiological Society and 1st Regional Congress of the Physiological Societies
poster
13.09.2013-15.09.2013
Rijeka, Hrvatska