engleski

HMW-GS as protein markers in differentiation of wheat cultivars

12 wheat (Triticum aestivum L.) cultivars differing in high molecular weight-glutenin subunits (HMW-GS) composition were collected from experimental field of the Agricultural Institute Osijek in 2009 and 2010. HMW-GS was analyzed by SDS-PAGE method and according to the catalogue of alleles the Glu1-score was calculated. The most frequently occurring HMW- GS were 2* 7+ 8/9 5 + 10. The proportion of HMW-GS in total extractable proteins of grains was analyzed by RP-HPLC and their average values in 2009 and 2010 were 8.91 and 9.55%, respectively. Comparing the cultivars, a several groups of related HMW-GS attributes were separated. Cultivar Divana as the Croatian bread improver with favorable HMW-GS composition (2* 7+9 5+10) and the highest HMW- GS proportion (11.98%) had a distinct position. In contrast, the Croatian yielding cultivar Sana was positioned on the opposite side due to unfavorable HMW-GS composition (2* 6+8 2+12) and the lowest HMW-GS proportion (6.24%). Cultivars Soissons, Felix, Aida and Ilirija with the highest Glu-1 score (10) belonged to the same cluster and their HMW-GS proportion varied between 8.90 and 10.01%. Cultivars Srpanjka and Golubica were due to higher proportion of HMW-GS (9.80 and 10.01%, respectively) and similar Glu-1 score (6 and 5, respectively) positioned very close.

wheat; HMW-GS; Glu-1 score; RP-HPLC

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