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Pregled bibliografske jedinice broj: 663719

Protein adsorption on various plasma-treated polyethylene terephthalate substrates


Recek, Nina; Jaganjac, Morana; Kolar, Metod; Milković, Lidija; Mozetič, Miran; Stana-Kleinschek, Karin; Vesel, Alenka
Protein adsorption on various plasma-treated polyethylene terephthalate substrates // Molecules, 18 (2013), 10; 12441-12463 doi:10.3390/molecules181012441 (međunarodna recenzija, članak, znanstveni)


Naslov
Protein adsorption on various plasma-treated polyethylene terephthalate substrates

Autori
Recek, Nina ; Jaganjac, Morana ; Kolar, Metod ; Milković, Lidija ; Mozetič, Miran ; Stana-Kleinschek, Karin ; Vesel, Alenka

Izvornik
Molecules (1420-3049) 18 (2013), 10; 12441-12463

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)

Sažetak
Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • CAB Abstracts
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • FSTA: Food Science and Technology Abstracts
  • INSPEC
  • MEDLINE


Citati